Increased de novo DNA Methylation Enzymes in Sperm of Individuals with Varicocele.

This article was published in Cell J (Yakhteh), Vol 23, No 4, 2021, on pages 389-396, corresponding author asked us to add another address (5. Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha, Qatar) to the fourth author's affiliation. The authors would like to apologies for any inconvenience caused.

Increased de novo DNA Methylation Enzymes in Sperm of Individuals with Varicocele.

Objective: Chronic genital heat-stress associated with varicocele leads to DNA hypo-methylation of spermatozoa. The objective of this study was comparing level of DNA methyl-transferases (DNMTs) in sperm of men suffering varicocele with fertile individuals.

Materials And Methods: In this case-control study, semen samples were obtained from 35 infertile men with varicocele (grade II or III) and 26 fertile men.

Comparison of main molecular markers involved in autophagy and apoptosis pathways between spermatozoa of infertile men with varicocele and fertile individuals.

Abnormal dilatation and tortuosity of the pampiniform plexus within the spermatic cord are termed varicocele which leads to impaired spermatogenesis due to heat-related oxidative stress and cell death. Previously, it was shown that both apoptosis and autophagy pathways were activated by heat in germ cells of mouse in vivo and in vitro. But, status of these pathways is not clear in chronic state of heat stress such as varicocele.

An association between sperm PLCζ levels and varicocele?

Purpose: We aimed to compare the expression of phospholipase C ζ (PLCζ), as one of the main sperm factors involved in oocyte activation, at both RNA and protein levels in fertile men and those with varicocele.

Methods: This study included 35 individuals with male factor infertility presenting primary infertility with grade II and III unilateral varicocele and 20 fertile men without varicocele. Semen parameters were assessed according to WHO 2010.

DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele.

This study aimed to assess and compare global DNA methylation (GDM) between fertile men and infertile men with varicocele. In addition, we evaluated the correlations between DNA methylation with reactive oxygen species (ROS), protamine deficiency, and DNA integrity. Semen samples were collected from 44 men with grades II and III varicocele, and 15 fertile men for assessment of semen parameters, DNA methylation, DNA fragmentation, oxidative stress, and protamine deficiency.

Semen parameters and chromatin packaging in microsurgical varicocelectomy patients.

Background: Varicocelectomy is considered as standard treatment for male infertility for clinical varicocele. The aim of this study is to address the effects of varicocelectomy on semen parameters, chromatin packaging, and pregnancy outcome.

Materials And Methods: This retrospective study was carried out between June 2006 and February 2011 on 145 infertile men with grade II or III varicocele.