Immunofluorescence in duodenal mucosa of children with acute enteritis due to a new virus.

Electron microscopy od duodenal mucosa from children with acute non-bacterial enteritis has shown virus particles in epithelial cells. Indirect immunofluorescent techniques applied to the same tissue showed virus antigen localized in the cytoplasm of epithelial cells of the villi. Specific IgM antibody was present in sera from infected patients as early as two days after the onset of symptoms.

Importance of a new virus in acute sporadic enteritis in children.

In a year-long survey of children admitted with acute enteritis to hospital in Melbourne, Australia, an aetiological agent was found in approximately 75% of patients. During most months of the year a newly described virus belonging to the family Reoviridae was detected by electron microscopy of faecal extracts from more than 50% of patients. Recovery of this virus reached a peak (73%) during the winter.

Bunyaviruses and Bunyaviridae.

A new family is described, the Bunyaviridae, which contains a single genus, Bunyavirus. The main characteristics of the family are as follows: single-stranded RNA, total molecular weight about 7 X 10(6) daltons, probably in three segments. Virions spherical, enveloped particles 90-100 nm in diameter.

Polyacrylamide gel electrophoresis of the genomes of two orbiviruses: D'Aguilar and Eubenangee.

The orbiviruses D'Aguilar and Eubenangee were shown to possess RNA genomes separable into ten segments by electrophoresis on polyacrylamide gels. Although there were minor differences in the molecular weights of corresponding genome segments of the two orbiviruses, the overall patterns were more similar to each other than to that of the reovirus type 3 genome.

Morphology and development of bovine ephemeral fever virus.

The development of the virus of bovine ephemeral fever in mouse brain has been studied by electron microscopy. The virus particles are bullet-shaped, 70 by 145 nm, and slightly tapered toward the rounded end. The outer envelope is closely apposed to an electron-dense shell, about 12 nm thick, but no other internal structure is visible.