Exchange of cytochrome b5 between phospholipid vesicles.

Detergent-extracted cytochrome b5, which binds readily to phosphatidylcholine vesicles, has been shown to exchange between vesicles by the following experiments. When preformed cytochrome b5-vesicle complexes are incubated with fresh vesicles a new species arises which sediments in the analytical ultracentrifuge with a rate intermediate between those of the original components. When the same mixture is subjected to free boundary electrophoresis no slow moving component corresponding to pure vesicles is seen.

Self-association of cytochrome b5 in aqueous solution. Gel filtration and ultracentirfugational studies.

The aggregation state of detergent-extracted cytochrome b5 was examined by gel filtration and sedimentation equilibrium ultracentrifugation. Both techniques indicated the presence of a mixture of monomer and octomer. The proportion of monomer was decreased as the buffer salt concentration was raised and was approximately 1 muM in 10 mM Tris acetate/0.

Effect of cytochrome b5 on the size, density, and permeability of phosphatidylcholine vesicles.

Cytochrome b5, isolated from rabbit liver by a procedure using detergent, was incubated with phosphatidylcholine bilayer vesicles at 37 degrees for 30 min. A comparison of a number of physical properties was made between the cytochrome b5-phosphatidylcholine complex (at a molar ratio of 1:1000) and the phosphatidylcholine vesicles. The binding of the protein to the vesicle caused no aggregation and no detectable change in Stokes radius of the vesicle as monitored by gel filtration.

Factors regulating amino acid release from extrasplanchnic tissues in the rat. Interactions of alanine and glutamine.

1. Factors regulating the release of alanine and glutamine in vivo were investigated in starved rats by removing the liver from the circulation and monitoring blood metabolite changes for 30 min. 2.

The effects of inhibition of gluconeogenesis on ketogenesis in starved and diabetic rats.

Experiments were performed in which the effects of inhibiting gluconeogenesis on ketone-body formation were examined in vivo in starved and severely streptozotocin-diabetic rats. The infusion of 3-mercaptopicolinate, an inhibitor of gluconeogenesis (DiTullio et al., 1974), caused decreases in blood [glucose] and increases in blood [lactate] and [pyruvate] in both normal and ketoacidotic rats.

The success of restorative dentistry?

Direct statistical evidence of the value of routine, periodic dental examination and treatment is lacking and inferences can only be drawn from surveys of population samples and groups of people. Except perhaps in areas of exceptionally favourable dentist: population ratios the restorative philosophy makes too high a demand on scarce professional resources for it to answer the treatment needs of populations. However, it was originally developed to benefit individual patients who sought dental care and the evidence at present suggests that it may postpone the loss of teeth.

Metabolic interactions of dichloroacetate and insulin in experimental diabetic ketoacidosis.

1. The infusion of sodium dichloroacetate into rats with severe diabetic ketoacidosis over 4h caused a 2mM decrease in blood glucose, and small falls in blood lactate and pyruvate concentrations. Similar findings had been reported in normal rats (Blackshear et al.

The metabolic effects of sodium dichloroacetate in the starved rat.

1. Sodium dichloroacetate (300mg/kg body wt. per h) was infused in 24h-starved rats for 4h.