Publications by authors named "Holley N Lewis"
Human bone marrow derived mesenchymal stem cells (hMSCs) hold great promise for regenerative medicine due to their multipotent differentiation capacity and immunomodulatory capabilities. Substantial research has elucidated mechanisms by which extracellular cues regulate hMSC fate decisions, but considerably less work has addressed how material properties can be leveraged to maintain undifferentiated stem cells. Here, we show that synthetic culture substrates designed to exhibit moderate cell-repellency promote high stemness and low oxidative stress-two indicators of naïve, healthy stem cells-in commercial and patient-derived hMSCs.
View Article and Find Full Text PDFDesign and efficacy of a single-use bioreactor for heart valve tissue engineering.
J Biomed Mater Res B Appl Biomater
February 2017
Article Synopsis
- - Heart valve tissue engineering has potential to help treat congenital heart disorders, but its clinical use is limited by scientific and regulatory issues, specifically the need for better bioreactor systems for seeding and conditioning valves.
- - Researchers developed a new bioreactor system and tested it on decellularized ovine aortic valves using human mesenchymal stem cells (hMSCs) to assess cell seeding and infiltration.
- - Results showed that negative and positive pressure conditioning led to cell infiltration into the valve leaflets, but the process only achieved partial cellular repopulation and maintained specific gene expression related to the cell lineage.