Publications by authors named "Holkem A"

Chlorella vulgaris has antioxidant, antimicrobial, and anti-inflammatory properties, as well as the probiotic that is important for keeping the intestinal microbiota balanced. The objective was to test the impact of supplementation with microalgae and/or probiotics on broiler chickens' performance, immunity, and intestinal microbiota. The experimental design was in randomized blocks in a 4x2 factorial scheme, with four levels of inclusion of C.

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Annual ryegrass (Lolium multiflorum Lam.) is one of the main weeds in subtropical cropping systems of Europe, Oceania and South America. Therefore, the hypothesis of this work is that the interaction between ammonium glufosinate and saflufenacil can be synergistic for ryegrass control.

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There is a current interest in healthy diets and supplements, indicating the relevance of novel delivery systems for plant extracts rich in bioactive compounds and probiotics. This simultaneous delivery system can be prospective for health. In this sense, investigating foods rich in bioactive compounds or supplemented by them for incorporating probiotics and some approaches to improve probiotic survivability, such as the choice of resistant probiotic strains or microencapsulation, is valuable.

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Bifidobacterium animalis subsp. lactis (BLC1) and proanthocyanidin-rich cinnamon extract (PRCE) have many beneficial health properties. However, they are very sensitive materials; co-encapsulation is one alternative to protect them.

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The association of probiotics and fruit extracts may influence the chemopreventive effect of colorectal cancer. In this context, antiproliferative activity was evaluated to select the best extracts that would be added probiotics, after addition of or in the extracts the antiradical and antioxidant activity, quinone reductase (QR) assay and apoptosis assay were evaluated. Four extracts were isolated: E1: rich in total phenolic compounds; E2: rich in water-soluble phenolic compounds; E3: rich in most apolar phenolic compounds and E4: rich in anthocyanins.

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Bioactive compounds are sensitive to many factors, and they can alter the sensory characteristics of foods. Microencapsulation could be a tool to provide protection and allow the addition of bioactives in new matrices, such as sugarcane juice. This study focused on producing and evaluating the potential function of probiotics and proanthocyanidin-rich cinnamon extract (PRCE), both in free and encapsulated forms when added to sugarcane juice.

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Guaraná is a native fruit of the Amazon rainforest, which presents high levels of phenolic compounds. However, these bioactive compounds may be unstable in food processing and gastrointestinal conditions. Thus, this work aimed to characterize guaraná seed extract (GSE) followed by microencapsulation using a spray-chilling method and with vegetable fat as carrier, as well as to evaluate the particles.

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In this work, sample preparation of carbon nanotubes (CNTs) for further determination of inorganic contaminants was investigated using a microwave-assisted wet digestion single reaction chamber system (MAWD-SRC). Analytes (Al, As, Ca, Cd, Co, Cr, Fe, La, Mg, Mo, Ni, Pb and Zn) were determined in CNTs by inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS, except for Al, Ca, Fe and Mg). Method parameters were evaluated, as the mass of CNT (25-300 mg), the temperature (220-270 °C) and the time (35-75 min) of irradiation program.

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