High-resolution MRI and micro-CT in an ex vivo rabbit anterior cruciate ligament transection model of osteoarthritis.

Objective: The aim of this study was to investigate the potential of using non-invasive, multi-modality imaging techniques to quantify disease progression in a rabbit model of experimentally induced osteoarthritis (OA).

Methods: High-resolution 4-T magnetic resonance imaging (MRI) and micro-computed tomography (micro-CT) techniques were implemented and validated in an ex vivo rabbit anterior cruciate ligament transection (ACLT) model of OA. A three-dimensional (3-D) rigid body registration technique was executed and evaluated to allow combined MR-CT analysis in co-registered image volumes of the knee.

Fundamental image quality limits for microcomputed tomography in small animals.

Small-animal imaging has become increasingly more important as transgenic and knockout mice are produced to model human diseases. One imaging technique that has emerged is microcomputed tomography (micro-CT). For live-animal imaging, the precision in the images will be determined by the x-ray dose given to the animal.

Cone-beam reprojection using projection-matrices.

This paper addresses reprojection of three-dimensional (3-D) reconstructions obtained from cone-beam scans using a C-arm imaging equipment assisted by a pose-determining system. The emphasis is on reprojecting without decomposing the estimated projection matrix (P-matrix) associated with a pose. Both voxel- and ray-driven methods are considered.

Image-based computational simulation of flow dynamics in a giant intracranial aneurysm.

Background And Purpose: Blood flow dynamics are thought to play an important role in the pathogenesis and treatment of intracranial aneurysms; however, hemodynamic quantities of interest are difficult to measure in vivo. This study shows that computational fluid dynamics (CFD) combined with computed rotational angiography can provide such hemodynamic information in a patient-specific and prospective manner.

Methods: A 58-year-old woman presented with partial right IIIrd cranial nerve palsy due to a giant carotid-posterior communicating artery aneurysm that was subsequently coiled.

MP3 compression of Doppler ultrasound signals.

The effect of lossy, MP3 compression on spectral parameters derived from Doppler ultrasound (US) signals was investigated. Compression was tested on signals acquired from two sources: 1. phase quadrature and 2.

In vitro investigation of calcium distribution and tissue thickness in the human thoracic aorta.

Atherosclerosis represents a significant cause of morbidity and mortality in the western world. Manifestations of atherosclerotic disease among the elderly include the development of raised lesions that often include calcified regions with material properties similar to bone. There is little information available about the amount and distribution of these calcified plaques within the human aorta, partly due to the difficulty in obtaining this information during in vivo studies.

An in vitro system for Doppler ultrasound flow studies in the stenosed carotid artery bifurcation.

To investigate the correlation between disease severity and Doppler spectral measurements in the carotid artery bifurcation, a unique in vitro system has been developed that mimics the human vasculature with respect to both anatomy and flow perfusion. Agar-based carotid phantoms are perfused with a blood-mimicking fluid using a computer-controlled pump and realistic pulsatile flow waveform. A three-axis translational stage allows the lumen to be interrogated with a 0.

Rapid small-animal dual-energy X-ray absorptiometry using digital radiography.

Although dual-energy X-ray absorptiometry (DEXA) is an established technique for clinical assessment of areal bone mineral density (BMD), the spatial resolution, signal-to-noise ratio, scan time, and availability of clinical DEXA systems may be limiting factors for small-animal investigations using a large number of specimens. To avoid these limitations, we have implemented a clinical digital radiography system to perform rapid area DEXA analysis on in vitro rat bone specimens. A crossed step-wedge (comprised of epoxy-based materials that mimic the radiographic properties of tissue and bone) was used to calibrate the system.

The radiographic quantitation of aortic valve calcification: implications for assessing bioprosthetic valve calcification in vitro.

Calcification of natural aortic and bioprosthetic heart valves is a poorly understood phenomenon that results in valvular obstruction and tissue failure. We describe a non-destructive quantitative computed microtomographic (QCT) technique for determining both calcium content and local calcium distribution within explanted valves. As a reference standard, a dual-energy x-ray absorptiometry (DEXA) system with an accuracy demonstrated to be within 1% of the true calcium mass of test material was used to obtain the total calcium content of 24 human aortic valve cusps recovered at autopsy from patients aged 51-80 years.

Bone mineral measurement of phalanges: comparison of radiographic absorptiometry and area dual X-ray absorptiometry.

With a standard, image-intensifier-based, digital radiographic system, high-spatial-resolution images of the hand were acquired for analysis of phalangeal bone mineral density with dual x-ray absorptiometry (DXA). Results with phalangeal DXA had precision of plus or minus 0.67% and accuracy of 4.

Flow patterns at the stenosed carotid bifurcation: effect of concentric versus eccentric stenosis.

Carotid stenosis severity is a commonly used indicator for assessing risk of stroke. However, the majority of individuals with severe carotid artery disease never suffer a stroke, and strokes can occur even with only mild or moderate stenosis. This suggests local factors (other than stenosis severity) at or near the carotid artery bifurcation may be important in determining stroke risk.

Chromosome analysis of workers occupationally exposed to radiation at the Sellafield nuclear facility.

Purpose: To investigate the relationship between stable chromosome aberration frequency in peripheral blood lymphocytes and occupational cumulative radiation exposure.

Materials And Methods: Cytogenetic analysis using G-banding was performed on peripheral blood lymphocyte cultures from 104 workers from the British Nuclear Fuels PLC facility at Sellafield, UK. The study group comprised 61 men with lifetime cumulative doses > 500 mSv, 39 men with minimal exposure (i.

The stimulation of vertebral and tibial bone growth by the parathyroid hormone fragments, hPTH-(1-31)NH2, [Leu27]cyclo(Glu22-Lys26)hPTH-(1-31)NH2, and hPTH-(1-30)NH2.

The native human parathyroid hormone, hPTH-(1-84), and certain carboxyl truncated analogs such as hPTH-(1-34) and even smaller fragments such as hPTH-(1-31)NH2, [Leu27]cyclo(Glu22-Lys26)hPTH-(1-31)NH2, and hPTH-(1-30)NH2 stimulate femoral trabecular and cortical bone growth in ovariectomized (OVX) rats. Here we show that when injected once daily for 6 weeks starting 2 weeks after OVX in doses of 1 or 2 nmol/100 g of body weight, hPTH-(1-31)NH2, [Leu27] cyclo(Glu22-Lys26)hPTH-(1-31)NH2, and hPTH-(1-34)NH2 prevented the loss of trabecular volume in the L5 vertebrae induced by OVX. In fact, by the end of the sixth week of injections (i.

Three-dimensional computed tomographic reconstruction using a C-arm mounted XRII: image-based correction of gantry motion nonidealities.

The image quality of 3D reconstructions produced using a C-arm mounted XRII depends on precise determination of the geometric parameters that describe the detector system in the laboratory frame of reference. We have designed a simplified calibration system that depends on images of a metal sphere, acquired during rotation of the gantry through 200 degrees. Angle-dependent shift corrections are obtained, accounting for nonideal motion in two directions: perpendicular to the axis of rotation and tangential to the circular trajectory (tau), and parallel to the axis of rotation (xi).

Quantitative angiographic blood-flow measurement using pulsed intra-arterial injection.

A technique for quantitative blood-flow measurement using a novel pulsed injection of radiographic contrast agent is reported. A pressurized source of contrast agent is interrupted by a rotary valve at rates ranging from 1 to 30 Hz, producing well-defined boli at the end of a catheter. The position of these boli can be recorded by a digital radiographic system and analyzed by one of several previously reported techniques, to produce quantitative measurements of blood velocity and flow rate throughout the cardiac cycle.

Anthropomorphic carotid bifurcation phantom for MRI applications.

Anthropomorphic carotid bifurcation flow phantoms that incorporate different stenotic geometries within the internal carotid artery have been developed. This technique produces high-fidelity, life-size vascular flow models that are compatible with magnetic resonance techniques. The models, in conjunction with a computer-controlled flow pump, address the need for a complex vascular geometry that can be used to verify magnetic resonance angiography (MRA) techniques that quantify stenosis severity and blood flow.

A three-dimensional cerebrovascular flow phantom.

We have constructed a life-sized fully three-dimensional (3D) rigid flow-through model of the cerebral vasculature. Average vessel diameters and lengths, taken from published values in the literature, were used to describe the geometry of our phantom; numerically controlled machining techniques were used to fabricate the model. Inflow to the phantom is provided through two internal carotid arteries and two vertebral arteries.

An investigation of the flow dependence of temperature gradients near large vessels during steady state and transient tissue heating.

Temperature distributions measured during thermal therapy are a major prognostic factor of the efficacy and success of the procedure. Thermal models are used to predict the temperature elevation of tissues during heating. Theoretical work has shown that blood flow through large blood vessels plays an important role in determining temperature profiles of heated tissues.

Characterization of common carotid artery blood-flow waveforms in normal human subjects.

Knowledge of human blood-flow waveforms is required for in vitro investigations and numerical modelling. Parameters of interest include: velocity and flow waveform shapes, inter- and intra-subject variability and frequency content. We characterized the blood-velocity waveforms in the left and right common carotid arteries (CCAs) of 17 normal volunteers (24 to 34 years), analysing 3560 cardiac cycles in total.

An analysis of the geometry of saccular intracranial aneurysms.

Background And Purpose: Our goal was to characterize the geometry of simple-lobed cerebral aneurysms and to find the absolute size of these lesions from angiographic tracings.

Methods: Measurements of angiographic neck width (N), dome height (H), dome diameter (D), and semi-axis height (S) were obtained from tracings of 87 simple-lobed lesions located at the basilar bifurcation (BB), middle cerebral (MCA), anterior communicating (AcomA), posterior communicating (PcomA), superior cerebellar (SCA), and posterior cerebral (PCA) arteries. The following ratios were analyzed as subgroups according to location and as a collective sample: dome diameter/dome height (D/H), dome height/neck width (H/N), dome diameter/neck width (D/N), and dome height/semi-axis height (H/S).

Techniques to alleviate the effects of view aliasing artifacts in computed tomography.

Due to practical limitations in data acquisition, 3-D computed tomography systems must attempt to provide rapid reconstructions of acceptable quality from a limited number of views. The use of convolution backprojection (CBP) for image reconstruction from an inadequate number of projections, results in view aliasing artifacts. In this paper we investigate different post-processing methods of alleviating the effects of view aliasing artifacts.

Application of dynamic computed tomography for measurements of local aortic elastic modulus.

A novel computed tomographic (CT) technique used for the instantaneous measurement of the dynamic elastic modulus of intact excised porcine aortic vessels subjected to physiological pressure waveforms is described. This system was comprised of a high resolution X-ray image intensifier based computed tomographic system with limiting spatial resolution of 3.2 mm-1 (for a 40 mm field of view) and a computer-controlled flow simulator.

Assessment of maintenance therapy with reduced doses of PTH(1-34) in combination with a raloxifene analogue (LY117018) following anabolic therapy in the ovariectomized rat.

This experiment was designed to evaluate the ability of a raloxifene analogue (RA), LY117018, with or without reduced dosing of human parathyroid hormone (hPTH)(1-34) to maintain gains in bone mass after a fully anabolic treatment regimen given to aging osteopenic rats. Six-month-old rats were ovariectomized (ovx) or sham-operated (sham). After 1 month, ovx rats were treated with an anabolic regimen consisting of subcutaneous hPTH(1-34) 80 microg/kg/day and oral raloxifene 3 mg/kg/day, each given 5 days/week for 3 months.

The addition of a raloxifene analog (LY117018) allows for reduced PTH(1-34) dosing during reversal of osteopenia in ovariectomized rats.

To test the hypothesis that an antiresorptive agent might reduce the dosing requirement for an anabolic drug during reversal of osteopenia due to estrogen deficiency, the following experiment was conducted in 6-month-old female rats. Ovariectomy or sham surgery was performed and the following six experimental groups were studied. Untreated (SHAM) or ovariectomized (OVX) animals served as control groups.

Accuracy of computational hemodynamics in complex arterial geometries reconstructed from magnetic resonance imaging.

Purpose: Combining computational blood flow modeling with three-dimensional medical imaging provides a new approach for studying links between hemodynamic factors and arterial disease. Although this provides patient-specific hemodynamic information, it is subject to several potential errors. This study quantifies some of these errors and identifies optimal reconstruction methodologies.