The three phases of vasospasm.

We propose the theory that prolonged cerebral vasospasm involves three phases: (1) the initial muscular contraction of the arterial wall; (2) a secondary injury to the artery that consists of endothelial desquamation with adherence of platelets to te denuded internal elastic lamina and mural thrombus formation; and (3) the repair process, which is the proliferative endarteropathy that has been observed in autopsy specimens. Cerebral ischemia can be the end product of any of these three conditions. We have postulated a possible subcycle in the overall scheme by which adherence of platelets to the denuded internal elastic lamina of the artery provide a continuously replenishing supply of spasmogenic chemical factors to the mural receptors and stimulate prolonged contraction of the muscular layer.

Locations of oligosaccharide chains in human alpha 1-protease inhibitor and oligosaccharide structures at each site.

Previous studies in this laboratory had shown that the alpha 1-protease inhibitor (alpha 1-PI) molecule contained two types of oligosaccharides, biantennary and triantennary. Cleavage of human alpha 1-protease inhibitor with CNBr resulted in three carbohydrate-containing fragments, I-III. Composition data revealed that each of these CNBr fragments contained one oligosaccharide chain.

Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum 775.

Cells of the marine fish pathogen Vibrio anguillarum 775 harboring a plasmid associated with virulence can grow unaffected in the presence of iron-binding compounds such as transferrin. In contrast, the growth of isogenic plasmidless derivatives is inhibited by the presence of iron chelators. Radioactive from (55Fe3+) uptake experiments indicate that this plasmid-linked ability of V.

Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum.

The transposon A sequence Tn1 containing the ampicillin resistance determinants was transposed from RP4 to a plasmid of the marine fish pathogen Vibrio anguillarum. Curing experiments in which plasmid loss was determined by analysis of the segregation of the ampicillin resistance phenotype showed the association of virulence with the specific V. anguillarum plasmid class.

Structure of the oligosaccharide chains in human alpha 1-protease inhibitor.

Two glycopeptides were obtained from alpha 1-protease inhibitor after extensive pronase digestion and chromatography on Bio-Gel P-10 and concanavalin A-Sepharose. these glycopeptides were characterized by compositional analysis and sequential exoglycosidase digestion followed at each step by methylation analysis. The partially methylated alditol acetates obtained were resolved by gas chromatography and identified by mass spectrometry.

In-Vitro and In-Vivo Effects of Aldicarb on Survival and Development of Heterodera schachtii.

Aqueous solutions of 5-500 mug/ml aldicarb inhibited hatching of Heterodera schachtii. Addition of hatching agents, zinc chloride, or sugarbeet root diffusate, to the aldicarb solutions did not decrease the inhibition of hatching. When cysts were removed from the aldicarb solufions and then treated for 4 wk in sugarbeet root diffusate, larvae hatched and emerged.

Efficacy of 1,2-Dibromo-3-chloropropane for Control of Meloidogyne javanica as Influenced by Concentration, Exposure Time and Rate of Degradation.

Laboratory experiments were conducted by applying 1,2-dibromo-3-chloropropane (DBCP) to sealed vials of soil infested with Meloidogyne javanica. A minimum initial concentration of 0.25 mug of DBCP/g of oven-dry soil killed all nematodes within 35 days.

Silent cholinesterase gene: variations in the properties of serum enzyme in apparent homozygotes.

The cholinesterase activity of the sera of 25 subjects diagnosed as homozygotes for the silent cholinesterase gene was studied by a sensitive enzymatic method employing several thiocholine esters and various inhibitors, and by disc electrophoretic, immunochemical, and chromatographic methods.(a) With one exception, the sera fell into two classes by all criteria. One class (type I, 16 cases) had no normal serum cholinesterase.