Enhancement of nematicidal potential through cloning and expression of gene from subsp. BTN7A strain.

A gene encoding chitinase from has been isolated after optimization of PCR conditions. It was cloned with two different prometers, T7 promoter of the pJET1.2/blunt cloning vector and the SP6 promoter of pGEM®-T Easy vector.

Evaluation of a nematode bio-product Dbx-20% against root-knot nematode Meloidogyne incognita affecting grapevine under field conditions.

A field trial was conducted in El-Shourouk Farm, El-Beheira governorate, western Nile valley, Egypt to determine the effectiveness of the commercial bio-product Dbx 1003 20% containing the nematode-trapping fungus Dactylaria brochopaga against root-knot nematode Meloidogyne incognita infesting grapevine variety Superior. Its effects on plant growth criteria and yield production were also investigated. The fungus was introduced to soil by either of two ways.