Two different types of intervening sequences in the glucoamylase gene from Aspergillus niger.

One single glucoamylase gene could be identified in the chromosomal DNA of Aspergillus niger by Southern blot analysis. This glucoamylase gene was isolated from a genomic library of A. niger DNA.

Glucoamylases G1 and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs.

By the use of glucoamylase-specific synthetic oligodeoxyribonucleotides and molecular cloning of cDNA synthesized from Aspergillus niger total poly(A) + RNA, the primary structure of the glucoamylase G1 mRNA was determined. Glucoamylase G1 is synthesized as a precursor of 640 amino acid residues containing a putative signal peptide of 18 residues, a short propeptide of six residues and the 616 residues long mature enzyme. In vitro translations of mRNA and immunoprecipitations with glucoamylase-specific antisera showed that two glucoamylase polypeptides are synthesized.

A short report from the project "Improved communication aids for people with speech impediments".

The project, initiated by Professor Olle Höök in 1976, is described. A review is given of different sub-projects, such as the development of an assessment form, the development of synthetic speech as a communication aid, communication aids for use with Bliss' symbol-system, development of individual communication aids, development of a play-inspired system for trying out control devices and finally development of play-materials for communication-training.