Does sildenafil citrate affect the pharmacokinetics of metformin in rats? Screening of mechanism through analytical and molecular docking approach.

Objective: In the present study, the effect of sildenafil on the pharmacokinetics of metformin was studied in experimental rats, and we also postulated the molecular mechanism by performing molecular docking studies.

Materials And Methods: Analysis of metformin and sildenafil (SIL) from rat plasma was done by high performance liquid chromatography. Optimum chromatographic separation and quantification of MET, SIL and Cetirizine was achieved on Phenomenex EVO C18 column with triethyl amine (0.

Optimization of High-Performance Liquid Chromatographic Method Using Central Composite Design for the Simultaneous Estimation of Ciprofloxacin and Loteprednol.

A simple, rapid, precise and accurate stability indicating high-performance liquid chromatographic (HPLC) method was developed for simultaneous quantification of Ciprofloxacin (CIP) and Loteprednol (LOT) along with their forced degradation products using central composite design (CCD) approach. CCD was prepared with three independent variables in a gradient HPLC method. In gradient program (GP) the ratio of phosphate buffer in the mobile phase was 70%, 75%, 80%, 85% and 90%, the pH of phosphate buffer was 2.