Antimicrobial resistance trends of non-fermenter Gram negative bacteria in Saudi Arabia: A six-year national study.

Background: Antimicrobial resistance (AMR) of non-fermenting Gram-negative bacteria (NFGNB) is increasingly recognized as urgent healthcare threat. Trend data on AMR of NFGNB in Saudi Arabia are either old or limited. The objective was to estimate the prevalence and resistance trends of isolated NFGNB in Saudi Arabia.

Rapid identification of from a brain abscess patient using MALDI-TOF-MS.

() is most frequently associated with human infections, including chronic bronchitis, pulmonary disease and brain abscesses. In general, causes infections in immunocompromised individuals and has been reported in clinical samples worldwide. However, the isolation and speciation of in the routine diagnostic microbiology laboratory are complicated and time consuming.

Assessment of nosocomial bacterial contamination in dental unit waterlines: Impact of flushing.

Purpose: This study evaluated the extent of bacterial contamination in water from dental unit waterlines (DUWLs).

Methodology: Water samples were collected (before flushing, 1 min post-flushing, and 3 min post-flushing) from 24 clinics (Group A: no disinfection, Group B: citric acid disinfectant) of a Government Dental College. Bacterial counts, identification, antibiotic sensitivity tests, determination of endotoxin levels, and scanning electron microscopy (to confirm the presence of biofilm) were performed.

Comparison of phenotypic and PCR methods for detection of carbapenemases production by Enterobacteriaceae.

Dissemination of carbapenem resistance via Enterobacteriaceae, particularly among and , is a major public health concern. Rapid methods for determining antimicrobial susceptibility are important to ensure adequate and appropriate use of antimicrobial agents and to limit the spread of these bacteria. In the current study, we compared the rapidity, sensitivity and specificity of traditional methods and molecular-based Xpert Carba-R PCR assay to identify sixty isolates, (26 and 34 ).

Comparison of different PCR methods for detection of Brucella spp. in human blood samples.

For detection of Brucella species by PCR four DNA extraction methods and four targets were compared using pure culture of Brucella melitensis and the best conditions were applied in clinical samples. It was found that the MagNA Pure LC method was the most efficient and sensitive method showing a positive PCR reaction with DNA extracted from as low as 25 and 100 CFU suspended in one ml blood and one ml water, respectively. Detection of Brucella spp.