[Species Identification of Pufferfish Products Using RAPD Analysis].

A simple and rapid method was developed to identify the source species of pufferfish products. Randomly amplified polymorphic DNA (RAPD) analysis was applied to identify 8 species of pufferfish. Commercial kits were used for DNA extraction and amplification.

[Determination method of ultra-high-intensity sweetener, advantame, in processed foods by HPLC and LC-MS/MS].

A simple method using HPLC and LC-MS/MS was developed for the determination of ultra-high-intensity sweetener, advantame, in processed foods. Advantame was extracted by dialysis, and cleaned up on a Sep-Pak Plus C18 cartridge, then determined by HPLC and LC-MS/MS. The recoveries from 5 kinds of processed foods fortified at the levels of 0.

Observed distribution of radiocaesium contamination in shiitake lots and variability of test results.

Shiitake mushrooms (Lentinula edodes) cultivated on bed-log are known to accumulate radiocaesium. Since the Fukushima-Diichi nuclear power plant accident (2011), the violation rate has been higher for log-cultivated shiitake than that for agricultural products or other foodstuffs. When testing shiitake mushrooms for radionuclide contamination, the validation of the sampling plan can be severely compromised by the heterogeneous contamination within shiitake lots.

[Determination method of linamarin in cassava products and beans by ultra high performance liquid chromatography with tandem mass spectrometry].

A rapid and simple method using ultra high performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) was developed for the determination of linamarin in cassava products and beans containing cyanogen compounds. Linamarin was extracted with acetonitrile-water (3 : 1) cleaned up using an amino solid-phase extraction cartridge, and then determined by UHPLC-MS/MS. The recoveries from cassava fortified at the levels of 10 μg/g and 100 μg/g were 96.

[Examination of radioactive contamination in foods].

Following the Fukushima nuclear plant accident in Mar. 2011, the examination of radioactive contamination in foods is being carried out in Nagoya. During the period between 30 Mar.

[Estimation of the intake of radioactive cesium based on analysis of total diet samples in Nagoya].

Food samples were purchased in Nagoya based on daily intake in the Tokai region, and prepared as total diet samples according to the market basket method. The contents of radioactive cesium (Cs) were determined by using a γ-ray spectrometer with a germanium semiconductor detector, and a committed effective dose was estimated. Radioactive Cs was not detected in samples collected in 2006 before the Fukushima nuclear plant accident.

[Species identification of animal hair present as a contaminant in food by PCR-APLP method].

A rapid, simple and inexpensive method was developed for identifying the species of animal hair present as a contaminant in food. A polymerase chain reaction-amplified product length polymorphism (PCR-APLP) assay was applied to identify hair from human and others (cat, dog, rabbit, rat and mouse) or livestock (pig, cattle, horse, sheep, goat and chicken). The PCR primers were designed to amplify partial sequences from the 16S rRNA gene to the NADH dehydrogenase subunit 1 (ND1) gene of mitochondrial DNA (mtDNA), which generate different length fragments for different animal species.

[Simultaneous determination of veterinary drugs in livestock foods and seafoods using liquid chromatography/tandem mass spectrometry].

A simultaneous determination of veterinary drugs in livestock food and seafood using liquid chromatography with tandem mass spectrometry (LC/MS/MS) was developed. Veterinary drugs were extracted with 95% acetonitrile. The solution was passed through a Florisil column, and the solvent was replaced with phosphate buffer.

[Analytical method for carbamate pesticides in processed foods by LC/MS/MS].

A rapid analytical method for the simultaneous determination of carbamate pesticides in processed foods was established by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). The pesticides were extracted from samples with acetonitrile using accelerated solvent extract equipment, except for the fine powder type spices, which were extracted in an ultrasonic bath. The crude extract was cleaned up with a multi-solvent GPC column (Shodex Asahipak GF-310 HQ) using acetonitrile as a mobile phase.

Determination and confirmation of five phenolic antioxidants in foods by LC/MS and GC/MS.

Identification and determination of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), nordihydroguaiaretic acid (NDGA), propyl gallate (PG) and tert-butylhydroquinone (TBHQ) by means of LC/MS and GC/MS were examined. These five phenolic antioxidants were detected as their pseudo-molecular ions [M-H]- by LC/MS using a Shim-pack FC-ODS column with drying gas. Moreover, BHA, BHT and TBHQ were detected based on their mass fragment ions by GC/MS.

[Determination of dl-alpha-tocopherol acetate and dl-alpha-tocopherol in foods by HPLC using post-column photochemical reaction].

A reliable analytical method for the simultaneous determination of dl-alpha-tocopherol acetate and dl-alpha-tocopherol in foods was established by HPLC using post-column photochemical reaction with UV and fluorescence detection. For low-fat food such as fruit juice and vegetable sauce, the tocopherols were extracted with methanol containing 0.1% ascorbic acid and the extract solution was injected into the HPLC.

[Determination of acrylamide in processed foods by column-switching HPLC with UV detection].

A simple and convenient analytical method for the determination of acrylamide in processed foods was established. Acrylamide was extracted with water in an ultrasonic bath. The extract was passed through an OASIS HLB cartridge and the eluate was injected into the HPLC system using a column-switching technique.