Electrochemical analysis of single nucleotide polymorphisms of p53 gene.

Single nucleotide polymorphisms (SNPs) of cancer repression gene p53 were analyzed electrochemically with ferrocenyl naphthalene diimide (1) as a hybridization indicator. The SNPs studied were the transition to A from G in the codon for amino acid at positions 175, 248 or 273 and the transversion to C from G in the codon for the amino acid at position 72. Thus, 20-meric oligonucleotides carrying the SNP site were used both as a sample and a probe with the latter immobilized on an electrode.

Optimization of silica-based media for antibody purification by protein A affinity chromatography.

Considering the large molecular size of IgG antibodies widely used for therapeutic applications, the pore size, pore volume and coupling density of silica-based media were optimized for the effective large-scale purification, using an antibody-protein A affinity system. Silica media, with average pore sizes from 70 nm to 140 nm and surface areas of 26-67 m(2)/g, were prepared and coupled with protein A. The static adsorption capacity and dynamic binding capacity of bovine and human IgG were measured at superficial liquid velocities ranging from 94 to 720 cm/h.

An anthracene derivative carrying ferrocenyl moieties at its 9 and 10 positions as a new electrochemically active threading intercalator.

A ferrocenyl anthracene derivative (FAN) was synthesized by connecting ferrocene moieties to the amino termini of its substitutents at 9 and 10 positions, aiming at an improved hybridization indicator. Spectrophotometric experiments revealed that FAN can bind to double stranded DNA (dsDNA) by threading intercalation. Evaluation of FAN in the dsDNA preference as hybridization indicator revealed that FAN has higher preference for dsDNA than ferrocenyl naphthalene diimdie (FND) reported previously.