Publications by authors named "Hirotoshi Shibata"
Background: In antibody-mediated nonhemolytic transfusion reactions, transfusion-related acute lung injury (TRALI) tends to occur typically within 2 hours after a blood transfusion. White cell antibodies or immune complexes have been frequently shown to be associated with the syndrome, although the mechanisms by which they induce TRALI are poorly understood. The aim of this study was to characterize soluble mediators that are released from cells at an early stage after immune stimulation.
View Article and Find Full Text PDFBackground: Antibodies to neutrophil antigens have been implicated in neonatal alloimmune neutropenia, autoimmune neutropenia, and transfusion-related acute lung injury. Most often, neutrophil-specific antibodies are directed toward human neutrophil antigen (HNA)-1 (Fcgamma receptor 3b) and HNA-2a (CD177) in these disorders.
Study Design And Methods: To detect the alloantibodies in the serum samples, a panel of cell lines was established in which the HNA-1a, HNA-1b (polymorphisms of HNA-1), or HNA-2a gene was transduced with a retrovirus vector to confer stable transgene expression in K562 cells that exhibited low background reactivity to human serum samples obtained from healthy donors in flow cytometric analysis.
The risk for hepatocellular carcinoma (HCC) among asymptomatic hepatitis C virus (HCV) carriers is not well understood. A community-based prospective study was conducted for over 8 years by record linkage to the Osaka Cancer Registry. The subjects were 1,927 individuals who were positive for anti-HCV through screening for second-generation HCV antibody (passive hemagglutination assay: >or= 2(12)) in voluntary blood donation.
View Article and Find Full Text PDF[Problems and an overview of organizations dealing with blood and blood products].
Nihon Naika Gakkai Zasshi
July 2004
Article Synopsis
- The study investigates the use of a modified coil planet centrifuge method to detect and measure mixed cell populations using monoclonal antibodies (MoAbs) instead of traditional polyclonal antisera, due to the latter's declining availability.
- The method was tested on packed red blood cells (RBCs) from different ABO blood types, chimeras, and patients who underwent ABO-incompatible hematopoietic progenitor cell transplantation (HPCT), revealing the ability to detect ABO chimerism as low as 0.1 percent.
- The findings confirm that the new method can analyze ABO chimerism and differentiate ABO variants with similar accuracy to existing methods, potentially improving early detection of relapse or rejection in HPCT patients.