High-Resolution Vertical Observation of Intracellular Structure Using Magnetically Responsive Microplates.

A vertical confocal observation system capable of high-resolution observation of intracellular structure is demonstrated. The system consists of magnet-active microplates to rotate, incline, and translate single adherent cells in the applied magnetic field. Appended to conventional confocal microscopes, this system enables high-resolution cross-sectional imaging with single-molecule sensitivity in single scanning.

Extreme Contrast of Postprandial Remnant-Like Particles Formed in Abetalipoproteinemia and Homozygous Familial Hypobetalipoproteinemia.

Background: Familial hypobetalipoproteinemia (FHBL) and abetalipoproteinemia (ABL) are rare inherited forms of hypolipidemia. Their differential diagnosis is important for predicting of the prognosis and selecting appropriate therapy.

Materials And Methods: Genetic analysis was performed in two patients with primary hypocholesterolemia born from consanguineous parents.

Parylene mobile microplates integrated with an enzymatic release for handling of single adherent cells.

An approach for manipulating single adherent cells is developed that is integrated with an enzymatic batch release. This strategy uses an array of releasable microfabricated mobile substrates, termed microplates, formed from a biocompatible polymer, parylene. A parylene microplate array of 10-70 μm in diameter can be formed on an alginate hydrogel sacrificial layer by using a standard photolithographic process.

A resettable dynamic microarray device.

This paper describes a simple reusable device that hydrodynamically traps a large number of beads in an array. Guiding pillars allow us to release the trapped beads by simply reversing the flow direction. The trap and reset operations are extremely simple, robust and highly efficient.

A dynamic microarray device for paired bead-based analysis.

In this study, we have developed a meander-shaped dynamic microfluidic technology that allows us to pair two different types of microbeads in a trapping site. The dynamic microfluidic technology comprises implemented modifications of a conventional dynamic microarray design such as: (i) the combination of a meander-shaped by-pass channel and a trapping channel with a hydrodynamic trapping site and (ii) line-symmetrical formation of the by-pass and trapping channels. Using these modifications, we have successfully trapped different types of sample in one trapping site, and constructed an array of paired beads of different type such as polystyrene beads or hydrogel beads made of agarose, collagen or alginate.

Precision-guided surgical navigation system using laser guidance and 3D autostereoscopic image overlay.

This paper describes a precision-guided surgical navigation system for minimally invasive surgery. The system combines a laser guidance technique with a three-dimensional (3D) autostereoscopic image overlay technique. Images of surgical anatomic structures superimposed onto the patient are created by employing an animated imaging method called integral videography (IV), which can display geometrically accurate 3D autostereoscopic images and reproduce motion parallax without the need for special viewing or tracking devices.