Aging Is Associated With Impaired Postprandial Response of Skeletal Muscle Protein Synthesis to High-Intensity Muscle Contraction in Mice.

The purpose of this study was to investigate whether aging alters the effect of nutritional status on contraction-induced muscle protein metabolism. In an overnight fasted or fed states, the right gastrocnemius muscle of young (3 months) and aged (24 months) male C57BL/6J mice was isometrically contracted via percutaneous electrical stimulation. The left gastrocnemius muscle served as a control.

Static light scattering study of complex formation between protein and neutral water-soluble polymer.

Complex formation of poly(N-isopropylacrylamide) (PNIPA) having a weight-average molecular weight of 1,720,000g/mol with human serum albumin (HSA), ovalbumin (OVA) and lysozyme (LYZ) was studied in an aqueous medium containing 0.01 M NaCl and adjusted to pH 3. The polymer-protein mixtures at different molar ratios (r(m)) were examined by static light scattering (SLS).

Light scattering study of complex formation between protein and polyelectrolyte at various ionic strengths.

Formation of protein-polyelectrolyte complexes (PPCs) between bovine serum albumin (BSA) and potassium poly (vinyl alcohol) sulfate (KPVS) was studied at pH 3 as a function of ionic strength. Turbidimetric titration was employed by a combination of dynamic light scattering (DLS) and electrophoretic light scattering (ELS). The formal charge (Z(PPC)) of the resulting PPCs at different ionic strengths were estimated from ELS data by assuming the free draining and the non-free draining model.