Publications by authors named "Hiroshi Koike"
Background: The Compress is designed to achieve bone formation and stability by applying pressure at the bone-implant interface, minimizing the likelihood of aseptic loosening, which is a complication of stem implants. Herein, we report two cases of implant failure using the Compress.
Case Presentation: Case 1 describes a 36 year-old Japanese man who underwent extraarticular tumor resection, Compress arthroplasty, and reconstruction with a gastrocnemius flap after preoperative chemotherapy for a secondary malignant giant cell tumor in the right distal femur.
Objective: In Japan, selumetinib is used in pediatric patients with neurofibromatosis type 1 (NF1) and symptomatic inoperable plexiform neurofibroma (PN). However, there have been no real-world reports on Japanese patients. In this study, we reported a single-center, short-term experience with selumetinib after its approval in Japan.
View Article and Find Full Text PDFDevelopment and performance evaluation of a microbiological method for screening and LC-MS/MS for conformation of sulfonamides in animal-derived foods.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
August 2024
Article Synopsis
- The study introduced a new microbiological method using a microtiter plate to detect 10 types of sulfonamide drugs in chicken muscles, eggs, and prawns, proving to be eco-friendly compared to traditional methods.
- The method involved a bioassay for initial residue detection, followed by confirmation and quantification using LC-MS/MS, achieving detection limits that meet Japan's minimum residual limits (MRLs).
- Quality control tests showed effective performance of the bioassay, and a survey found sulfamethoxazole in one prawn sample out of 91 tested, demonstrating the method's practical application.
Article Synopsis
- Polycystic ovary syndrome (PCOS) is a common disorder affecting women's reproductive health, but its causes and best treatments are not fully understood.
- Research indicates that cellular senescence, where cells permanently stop dividing due to stress, plays a role in PCOS, particularly through inflammatory factors released by senescent cells.
- A study found that the senolytic drug combination dasatinib and quercetin (DQ) reduces senescent cell accumulation and improves ovarian health in PCOS models, suggesting a potential new treatment for the condition.
Article Synopsis
- * A study using a mouse model showed that changes in gut microbiota appear before puberty, with reproductive symptoms arising in adolescence and metabolic symptoms emerging in young adulthood.
- * Cross-fostering experiments revealed that nurturing from normal mothers leads to less severe PCOS-like traits and gut microbiome changes in PNA offspring, suggesting both prenatal and postnatal environments are important for PCOS development and could be potential prevention targets.
Article Synopsis
- The study focuses on developing a screening method to detect cereulide, a toxin from Bacillus cereus, in fried rice, which is important for identifying food poisoning cases even when the bacteria itself may not be found.
- The researchers used the QuEChERS extraction method along with LC-MS/MS to identify and quantify cereulide, achieving accuracy levels between 88% and 89%, and establishing a limit of quantification at 2 µg/kg.
- Results indicated that the method effectively removed interfering compounds and provided reliable quantification, meeting Japanese validation guidelines, thus showing potential as a dependable tool for checking fried rice for cereulide contamination.
Article Synopsis
- Foreign body granulomas (FBGs) can develop from chronic inflammation due to leftover foreign materials, with intraperitoneal foreign body granulomas (IPFBGs) commonly arising from surgical materials but rarely from food residue.
- The case involves a 74-year-old male who experienced anastomotic leakage after rectal cancer surgery, leading to difficulty distinguishing his IPFBG caused by food from peritoneal dissemination identified through imaging.
- The findings suggest that food residue can indeed trigger IPFBG, highlighting the importance of considering this condition in treatment decisions for peritoneal nodules.
Simultaneous determination of five carbapenems, highly polar antibiotics, in milk by LC-MS/MS.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
February 2024
Article Synopsis
- A new method using LC-MS/MS was developed to simultaneously detect five types of carbapenem antibiotics (biapenem, doripenem, ertapenem, imipenem, meropenem) in both raw and pasteurized bovine milk samples.* -
- The method involved chromatographic separation on a specialized column, extraction processes to remove lipids and proteins, and validation according to Japanese guidelines, showing good accuracy and reproducibility.* -
- Testing of 60 milk samples from Tokyo found no traces of the carbapenems, indicating the method's effectiveness for routine monitoring of these antibiotics in milk.*
Article Synopsis
- The study examines how proton pump inhibitors (PPIs) and potassium-competitive acid blockers (P-CABs) affect the absorption and efficacy of the oral drug pazopanib in patients with soft tissue sarcoma.
- Researchers analyzed medical records of eight patients who were treated with pazopanib, considering those who also used PPIs/P-CABs.
- Results indicated that while the use of PPIs/P-CABs did not significantly affect the time to treatment failure, it led to less frequent dose reductions/interruption of pazopanib and milder neutropenia, suggesting a potential decrease in pazopanib's pharmacological activity.
Article Synopsis
- - Young female cancer patients undergoing chemotherapy with cyclophosphamide (Cy) risk developing primary ovarian insufficiency (POI) due to ovarian damage.
- - The study found that cellular markers of senescence were heightened in granulosa cells after Cy treatment, suggesting a link between senescence and POI, which was reduced with the senolytics dasatinib and quercetin (DQ).
- - DQ treatment showed potential in improving ovarian health and fertility in mice with Cy-induced POI by enhancing ovulation response and extending reproductive lifespan, highlighting the importance of targeting senescent cells.
Article Synopsis
- * The study found that ipriflavone reduced KIAA1199 activity, which is linked to OA progression, and preserved vital components like hyaluronan (HA) in cartilage tissue.
- * Results suggest that ipriflavone could serve as a potential treatment for OA by inhibiting factors that lead to its development and progression.
Roles of endoplasmic reticulum stress in the pathophysiology of polycystic ovary syndrome.
Front Endocrinol (Lausanne)
March 2023
Article Synopsis
- Polycystic ovary syndrome (PCOS) is a common endocrine disorder affecting about 15% of reproductive-age women, often leading to infertility due to anovulation.
- Recent research highlights the significant role of endoplasmic reticulum (ER) stress in PCOS, caused by an overload of misfolded proteins in the ER, which can trigger cellular responses that either restore function or lead to cell death.
- Studies show that ER stress pathways are activated in the ovaries of both mice and humans with PCOS, linking hyperandrogenism to these stress responses, suggesting that targeting ER stress could offer new treatment options for PCOS.
Article Synopsis
- A rapid analytical method was validated for detecting 20 different veterinary drugs in poultry muscle, eggs, and raw milk using LC-MS/MS technology.
- The method involves extracting analytes with acetonitrile and then purifying them with magnesium sulfate, trisodium citrate, and sodium chloride.
- In tests, 17 out of 20 drugs were detectable in chicken and duck muscle, while all drugs were found in eggs and raw milk, suggesting further monitoring for veterinary drugs is needed as some residues were detected below the quantification limits.
Validation and application of an immunochromatographic test to detect four macrolides and two lincosamides in raw cow milk.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
April 2023
Article Synopsis
- This study evaluated the Charm QUAD2® Test, an immunochromatographic method, for detecting residual macrolides and lincosamides in raw cow's milk, showing high selectivity and a 0% false-positive rate.
- The test met validation parameters according to European Commission standards, demonstrating low detection limits for various antibiotics that are below most maximum residue limits in Japan, except for lincomycin.
- Results from the test correlated well with traditional chemical and microbiological methods, indicating its potential for routine food safety analysis in milk.
Article Synopsis
- The study aimed to develop a new technique for detecting Clostridium perfringens enterotoxin (CPE) in human stool, which is important for diagnosing food poisoning.
- It involved spiking CPE into artificial gut fluid and using stable isotope-labelled peptides alongside liquid chromatography-tandem mass spectrometry (LC-MS/MS) for analysis.
- The developed method successfully quantified CPE with good recovery rates and low standard deviations, making it a reliable tool for future assessments of CPE.
Article Synopsis
- A 73-year-old woman was diagnosed with gastric cancer and acute cholecystitis, leading to surgery that included distal gastrectomy and cholecystectomy.
- Post-surgery, the cancer was confirmed to be adenosquamous cell carcinoma, a rare and aggressive type, requiring chemotherapy.
- Despite treatment efforts, including multiple chemotherapy regimens, the patient's condition worsened, and she passed away 10 months after surgery.
Article Synopsis
- The study investigates the surgical outcomes and complications of removing deep-seated nodular plexiform neurofibromas in patients with neurofibromatosis type 1, based on a review of cases from 2015 to 2021.
- Surgical methods included enucleation to preserve nerves and en bloc resection when nerves were entrapped, involving 24 tumors in 15 patients.
- Post-surgery, all patients experienced symptom resolution, though some faced new but mild neurological deficits, suggesting the procedure is generally safe with a low risk of severe complications.
Background: The treatment of choice for desmoid-type fibromatosis (DF) has been changed to active surveillance (AS). However, few studies have reported clinical outcomes of AS modality in Asian countries. This study aimed to clarify the significance of AS as a DF treatment modality.
View Article and Find Full Text PDFArticle Synopsis
- Endoplasmic Reticulum (ER) Stress
- : ER stress in granulosa cells is linked to the dysfunction observed in Polycystic Ovary Syndrome (PCOS), suggesting that it may impact ovarian health.
- Notch Signaling Activation
- : Notch signaling pathways are activated in response to ER stress in granulosa cells, leading to increased expression of Notch2 and related transcription factors, which are associated with abnormal expansion of the cumulus-oocyte complex (COC).
- Regulation of COC Expansion
- : Inhibiting Notch signaling can reverse the effects of ER stress on COC expansion both in cultured human cells and in mouse models, indicating that Notch
Anticancer drugs and molecular targeted therapies are used for refractory desmoid-type fibromatosis (DF), but occasionally cause severe side effects. The purpose of this study was to identify an effective drug with fewer side effects against DF by drug repositioning, and evaluate its efficacy. FDA-approved drugs that inhibit the proliferation of DF cells harboring S45F mutations of CTNNB1 were screened.
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- Researchers used a specialized LC-MS/MS method to analyze 127 honey samples from Tokyo for residual acaricides between April 2015 and March 2021.
- Amitraz was found in 85 samples at levels ranging from 1.1 to 34.1 μg/kg, while propargite was detected in 3 samples at 2.4 to 3.8 μg/kg, but all results were within Japanese maximum residue limits (MRLs).
- The consistent presence of amitraz over the years indicates proper usage in beekeeping, while the detection of propargite for the first time in 2020 may point to a changing trend in acaricide applications in Japan.
Article Synopsis
- Hyaluronan (HA) is important for maintaining cartilage, but its changes in osteoarthritis (OA) and osteonecrosis of the femoral head (ONFH) need more research.
- The study focused on how KIAA1199, a hyaluronidase, affects HA metabolism in OA and ONFH by analyzing human cartilage samples.
- Findings revealed that KIAA1199 is overexpressed in OA, leading to changes in HA levels, while ONFH showed lower KIAA1199 expression and a less active HA metabolism.
Article Synopsis
- * Ipriflavone was identified as a potential drug, restoring staining of cartilage cells and increasing extracellular matrix formation in a dose-dependent manner.
- * In both cell experiments and mouse models of arthritis, Ipriflavone improved symptoms and decreased hyaluronan levels, suggesting it may be an effective treatment for inflammatory arthritis.
Article Synopsis
- - The patient, a 71-year-old man, had pancreatic cancer and underwent surgery known as subtotal stomach-preserving pancreaticoduodenectomy along with D2 lymphadenectomy.
- - After 38 months, a CT scan revealed a 10-mm mass in his left lung, leading to concerns about either lung metastasis from his pancreatic cancer or a new primary lung cancer.
- - Following a partial resection of the lung, it was confirmed that the mass was a metastasis from the pancreatic cancer, but 9 years later, the patient has not experienced any recurrence.
Article Synopsis
- - Fibrous dysplasia is a rare condition that can cause fractures, pain, and other disabilities but lacks effective medical treatments; surgery is sometimes impractical due to the extent of the lesions.
- - A 27-year-old woman with right buttock pain was diagnosed with cystic fibrous dysplasia, and initial tests showed no associated syndromes or abnormal blood values, although her acid phosphatase level was elevated.
- - The patient was treated with denosumab, resulting in pain relief and improved bone density, and this positive effect lasted for 9 months after the treatment ended, highlighting the potential of denosumab for off-label use in fibrous dysplasia cases.