Detection of Mycoplasma wenyonii and "Candidatus Mycoplasma haemobos" from Japanese Black breeding cows in Kyushu and Okinawa region, southern part of Japan.

This study aimed to elucidate the epidemiological status of hemoplasma infection and investigate the interaction between Theileria orientalis and hemoplasmas in Japanese Black breeding cows raised in the Kyushu and Okinawa regions. Blood samples were collected from 400 cows from 80 different farms in eight prefectures (five samples per farm and 10 farms per prefecture). Mycoplasma wenyonii (Mw), "Candidatus Mycoplasma haemobos" (CMh), and T.

Effects of live yeast supplementation on serum oxidative stress biomarkers and lactation performance in dairy cows during summer.

This study aimed to evaluate the effects of live yeast (Saccharomyces cerevisiae) (LY) supplementation on serum oxidative stress biomarkers, antioxidant vitamin levels, and lactation performance in dairy cows during summer. A total of 16 lactating cows weighing 707.5 ± 13.

Validation of a novel timed artificial insemination protocol in beef cows with a functional corpus luteum detected by ultrasonography.

This study aimed to clarify the feasibility of a novel timed artificial insemination (TAI) protocol using ultrasonography, and to determine the associations between the ovarian component and fertility. In Experiment 1, 272 Japanese Black cows with a corpus luteum (CL) ≥ 18 mm in diameter were divided randomly into either the TRT group (134 cows that were administered gonadotropin-releasing hormone [GnRH] 56 h [day 2] after prostaglandin F [PGF] administration [day 0], followed by TAI 16-20 h later) or the CN-1 group (138 cows that were administered PGF followed by AI after estrus detection). In addition, the CN-2 group was designated for 306 cows given PGF and inseminated after estrus detection in the past two years at the same farms.

Seasonal changes in serum oxidative stress biomarkers in dairy and beef cows in a daytime grazing system.

This study aims to evaluate the oxidative stress during hot summer season using serum oxidative stress biomarkers and elucidate the effects of serum antioxidant vitamin levels in dairy and beef cows in a daytime grazing system. Blood samples were collected once a month from eight Holstein Friesian (HF) and 10 Japanese Black (JB) cows from November 2013 to October 2014. Serum values of derivatives of reactive oxygen metabolites (d-ROMs) tended to be higher in March in both breeds and those in HF cows were kept at higher (P<0.

In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens.

First-generation cephalosporins such as cefazolin (CEZ) have been widely used for mastitis treatment in dairy cattle. However, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. Nisin A, a bacteriocin produced by Lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years.

Histopathological changes in the pancreas of cattle with abdominal fat necrosis.

The association between pancreatic disorder and abdominal fat necrosis in cattle remains unclear. The pancreases of 29 slaughtered cattle with or without fat necrosis were collected to investigate pathological changes. Japanese Black (JB) cattle were classified into the FN group (with abdominal fat necrosis; n=9) and N group (without fat necrosis; n=5).

The association of culling and death rate within 30 days after calving with productivity or reproductive performance in dairy herds in Fukuoka, Southern Japan.

The incidence of peripartum disorders in dairy herds negatively influences productivity and reproductive performance. Concrete data from local areas are helpful for explaining the importance of peripartum management to dairy farmers. This study was conducted to clarify the association of culling and death rate within 30 days after calving with productivity or reproductive performance in 179 dairy herds in Fukuoka, Southern Japan.

Effects of ozonated autohemotherapy on the antioxidant capacity of Thoroughbred horses.

The performance of horses undergoing regular intense exercise is adversely affected by oxidative stress. Thus, it is important to increase antioxidant production in horses in order to reduce oxidative stress. Ozonated autohemotherapy (OAHT) reportedly promotes antioxidant production.

Accumulation of xanthophylls from the phaffia yeast (Xanthophyllomyces dendrorhrous) in calves.

An investigation into the absorption and accumulation of carotenoids from phaffia yeast in two to three-week-old calves was carried out. Carotenoid contents of the control cattle (n=1) were 615.0 ng/g in the liver, 263.

Evaluation of anti-Müllerian hormone in a dog with a Sertoli cell tumour.

Background: Testicular tumours are common in elderly male dogs, and Sertoli cell tumours (SCTs) are among the most common. An increase in blood estradiol concentration is often seen in canine SCTs, but such measurements do not necessarily correlate with the clinical signs.

Case Report: A 6-year-old male Pembroke Welsh corgi was referred for nonpruritic alopecia.

Nectin4 is an epithelial cell receptor for canine distemper virus and involved in neurovirulence.

Canine distemper virus (CDV) uses signaling lymphocyte activation molecule (SLAM), expressed on immune cells, as a receptor. However, epithelial and neural cells are also affected by CDV in vivo. Wild-type CDV strains showed efficient replication with syncytia in Vero cells expressing dog nectin4, and the infection was blocked by an anti-nectin4 antibody.

Effect of sugar supplementation on rumen protozoa profile and papillae development in retarded growth calves.

The effect of sugar supplementation with 1 g/kg BW twice a week for eight weeks on rumen protozoa was determined in ten retarded growth calves. Rumen juice was sampled by abdominal paracentesis during the experiment. Papillae development of rumens excised by experimental laparotomy was macro- and micromorphologically determined before and after sugar supplementation in a selected calf.

Rumen ciliates from Tanzanian short horn zebu cattle, Bos taurus indicus, and the infraciliature of Entodinium palmare n.sp. and Enoploplastron stokyi (Buisson, 1924).

Rumen ciliates of ten Tanzanian short horn zebu cattle were examined. A total of 15 genera and 46 species were identified, including a new Entodinium species. The ciliate density was 22.

Relationship between plasma vitamin C and serum diagnostic biochemical markers in lactating cows.

We investigated the relationship between plasma vitamin C concentration and serum levels of some diagnostic biochemical markers in 118 lactating Holstein cows. Blood sample was collected once from each cow and we measured the plasma vitamin C concentration and the serum levels of glucose, beta-hydroxybutyrate, free fatty acids, triacylglycerol, total cholesterol, albumin, total bilirubin, alkaline phosphatase, aspartate aminotransferase and gamma-glutamyltransferase. The regression of plasma vitamin C with each serum diagnostic biochemical marker indicated that the vitamin C concentration significantly decreased as glucose, alkaline phosphatase or aspartate aminotransferase level increased and as total cholesterol or albumin concentration decreased.

Surgical management of combined hydrocephalus, syringohydromyelia, and ventricular cyst in a dog.

Combined hydrocephalus, syringohydromyelia, and a ventricular cyst were found by magnetic resonance imaging in a 7-month-old, male miniature dachshund with gait abnormalities and altered mentation. Clinical signs did not improve with prednisone therapy. Ventriculoperitoneal shunting improved the clinical signs and anatomical abnormalities.

Seasonal changes in serum vitamin A, vitamin E and beta-carotene concentrations in Japanese Black breeding cattle in Hyogo prefecture.

The influence of the season on serum vitamin A, E and beta-carotene concentrations was studied in Japanese Black breeding cattle. Blood samples were collected from 13 cows once a month for a year to determine the serum vitamin concentrations. Mean serum concentrations (+/- SE) of retinol, alpha-tocopherol and beta-carotene were 21.