Publications by authors named "Hiromichi Kato"
Article Synopsis
- The novel esterase gene lipP1, found in the extremely halophilic archaeon Haloarcula japonica, encodes the enzyme HjEstP1, classified as a family IV esterase with specific conserved motifs.
- HjEstP1 was expressed in its original strain, purified, and characterized, revealing optimal activity at a pH of 6.0 and temperatures above 60 °C.
- The enzyme demonstrated increased activity with higher NaCl concentrations, peaking at over 4.5 M, and preferentially hydrolyzed pNP and glycerol esters with short-chain fatty acids, marking it as the first esterase identified from an extremely halophilic archaeon through homologous expression.
Protein-protein interactions (PPIs) are crucial for various biological processes. Epstein-Barr virus (EBV) proteins typically form complexes, regulating the replication and persistence of the viral genome in human cells. However, the role of EBV protein complexes under physiological conditions remains unclear.
View Article and Find Full Text PDFPurification and some properties of cathepsin H from rabbit skeletal muscle.
Int J Biochem Cell Biol
April 2003
Rabbit muscle cathepsin H classified as an aminoendopeptidase was purified and its properties were investigated to clarify its contribution to the proteolysis of postmortem muscle. The purification was performed by ammonium sulfate fractionation and successive chromatographies on Sephadex G-75, phosphocelluose, DEAE-Sephadex A-50 and Sephadex G-100. The purified enzyme gave a single protein band on SDS-PAGE.
View Article and Find Full Text PDF