Early induction of moderate hypothermia suppresses systemic inflammatory cytokines and intracellular adhesion molecule-1 in rats with caerulein-induced pancreatitis and endotoxemia.

Objectives: To evaluate the systemic effects of moderate hypothermia (MH) and the timing of induction on acute pancreatitis (AP) and endotoxemia in rats.

Methods: The effects of MH were compared in 4 groups, that is, sham group (38 degrees C), control group (38 degrees C), early MH group (32 degrees C on administration of lipopolysaccharide [LPS]), and delayed MH group (32 degrees C 1 hour after LPS). AP and endotoxemia were induced by intramuscular injection of caerulein and intraperitoneal injection of LPS.

Pharmacokinetic study of ethanol and its metabolites during ethanol infusion.

We examined the effects of blood ethanol level on the disposition of acetaldehyde and acetate. Rabbits were divided into two groups (1 mg/ml and 2 mg/ml groups). An ethanol saline solution was injected intravenously into these rabbits at 1.

Pharmacokinetic study of ethanol and its metabolites in culture cells.

We examined the disposition of ethanol, acetaldehyde and acetate in culture cells. Rat hepatoma-derived cells, H4IIE, were grown in T-shaped flasks to confluence in minimal essential medium supplemented with 5% fetal bovine serum. For the experimental group, the medium in the flask was replaced with a medium containing a 0, 4, 8 or 20 mM ethanol solution.

Michaelis-Menten elimination kinetics of acetaldehyde during ethanol oxidation.

Background: Acetaldehyde (AcH) is a toxic metabolite of ethanol (EtOH). The pharmacokinetics of blood AcH during EtOH oxidation was studied with or without the administration of aldehyde dehydrogenase 2 inhibitor (cyanamide) in rabbits.

Methods: An bolus of EtOH saline solution (0.