Evaluation of the repeated-dose liver, bone marrow and peripheral blood micronucleus and comet assays using kojic acid.

The repeated-dose liver micronucleus assay has the potential to detect liver carcinogens and could be integrated into general toxicological studies. To assess the performance of this assay, kojic acid was tested in 14-day and 28-day liver micronucleus assays. We evaluated the incidence of micronucleated cells in liver, bone marrow and peripheral blood and performed comet assays in both the liver and peripheral blood (comet assay was performed only for 14-days).

Optimum design for glass fiber-reinforced composite clasps using nonlinear finite element analysis.

The purpose of this study was to design an optimum glass fiber-reinforced composite (FRC) clasp. Three-dimensional finite element models were constructed of FRC circumferential clasp arms and an abutment tooth. The basic clasp arm was half-oval, without a taper, 2.

DNA binding-dependent glucocorticoid receptor activity promotes adipogenesis via Krüppel-like factor 15 gene expression.

Glucocorticoids, such as dexamethasone, have been used as in vitro inducers of adipogenesis. However, the roles of the glucocorticoid receptor (GR) in adipogenesis have not been well characterized yet. Here, we show that inhibition of GR activity using the GR antagonist RU486 prevents human mesenchymal stem cell and mouse embryonic fibroblast (MEF) differentiation into adipocytes.

Stress analysis of clasps made of glass fiber-reinforced composite material using three-dimensional finite element method: influence of shape in cross and longitudinal sections of circumferential clasp arms.

The purpose of this study was to investigate the effects of different cross-sectional shapes and presence of taper on stress distribution in clasp arms made of glass fiber-reinforced composite (FRC) material. Stress analysis of clasps was performed under a constant load (5 N) using a three-dimensional finite element method with due consideration to the anisotropy of unidirectional FRC material. Results were then compared with clasp arms made of metal- an isotropic material.

Hepatocyte growth factor attenuates airway hyperresponsiveness, inflammation, and remodeling.

Hepatocyte growth factor (HGF) is known to influence a number of cell types and their production of regulatory cytokines. We investigated the potential of recombinant HGF to regulate not only the development of allergic airway inflammation and airway hyperresponsiveness (AHR), but also airway remodeling in a murine model. Administration of exogenous HGF after sensitization but during ovalbumin challenge significantly prevented AHR, as well as eosinophil and lymphocyte accumulation in the airways; interleukin (IL)-4, IL-5, and IL-13 levels in bronchoalveolar lavage (BAL) fluid were also significantly reduced.