Publications by authors named "Hiromasa Maehira"
Article Synopsis
- Developing better engineering techniques for G-protein-coupled receptors (GPCRs) is crucial due to their role in drug targeting and signaling pathways.
- A high-throughput screening method was established using an in vitro transcription-translation (IVTT) system to synthesize human endothelin receptor type-B (ETBR) within a phospholipid nanodisc, which improved its ability to bind the peptide hormone endothelin-1 (ET-1).
- The study successfully demonstrated that both ETBR and the unresolved receptor ETAR could be functionally synthesized and screened for mutations using ribosome display in this system, indicating the potential for gene screening and directed evolution of GPCRs.
