Fluorophotometric determination of histone with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone-manganese(II) complex and its characterization.

A simple fluorophotometric method for the determination of histone has been developed. This method involves a fluorescence quenching reaction that results in the formation of a complex of manganese(II), 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone (TFCPF), and histone in a non-ionic surfactant micellar medium. The calibration curve was found to be linear in the range of 0.