Publications by authors named "Hiroki Ishikida"
Protein Expr Purif
July 2015
Article Synopsis
- - Galectins are a group of proteins that bind to specific sugar molecules (β-galactoside sugars) due to their unique amino acid structure, and are used to understand how these interactions work outside of cells.
- - Purifying galectins can be straightforward but becomes challenging with mutated versions that have lower sugar binding affinity; however, the galectin LEC-6 from the worm C. elegans maintains strong binding even when mutated.
- - In research, various mutant forms of mouse galectin-1 were created to test their sugar binding ability; most mutants had low affinity for one sugar but could still be purified using another specific sugar (Galβ1-4Fuc), suggesting this could be a useful
