Growth of Bifidobacterium pseudocatenulatum in medium containing N-acetylsucrosamine: enzyme that induces the growth of this bacterium via degradation of this disaccharide.

Bifidobacterium pseudocatenulatum grows well in the early stages of cultivation in medium containing sucrose (Suc), whereas its growth in medium containing the analogue disaccharide N-acetylsucrosamine (SucNAc) tends to exhibit a considerable delay. To elucidate the cause of this phenomenon, we investigated the proliferation pattern of B. pseudocatenulatum in medium containing D-glucose (Glc) and SucNAc and identified the enzyme that degrades this disaccharide.

Enzymatic Synthesis and Structural Confirmation of Novel Oligosaccharide, D-Fructofuranose-linked Chitin Oligosaccharide.

Utilizing transglycosylation reaction catalyzed by β- -acetylhexosaminidase of , β-D-fructofuranosyl-(2↔1)-α- , ´diacetylchitobioside (GlcNAc -Fru) was synthesized from -acetylsucrosamine and , ´-diacetylchitobiose (GlcNAc ), and β-D-fructofuranosyl-(2↔1)-α- , ´, ´´-triacetylchitotrioside (GlcNAc -Fru) was synthesized from GlcNAc -Fru and GlcNAc . Through purification by charcoal column chromatography, pure GlcNAc -Fru and GlcNAc -Fru were obtained in molar yields of 33.0 % and 11.

Utilization of sucrose and analog disaccharides by human intestinal bifidobacteria and lactobacilli: Search of the bifidobacteria enzymes involved in the degradation of these disaccharides.

The majority of oligosaccharides used as prebiotics typically consist of a combination of 3 kinds of neutral monosaccharides, d-glucose, d-galactose, and d-fructose. In this context, we aimed to generate new types of prebiotic oligosaccharides containing other monosaccharides, and to date have synthesized various oligosaccharides containing an amino sugar, uronic acid, and their derivatives. In this study, we investigated the effects of 4 kinds of sucrose (Suc) analog disaccharides containing d-glucosamine, N-acetyl-d-glucosamine, d-glucuronic acid, or d-glucuronamide as constituent monosaccharides, on the growth of 8 species of bifidobacteria and 3 species of lactobacilli isolated from the human intestine.

Chemoenzymatic synthesis of sucuronic acid using d-glucurono-6,3-lactone and sucrose as raw materials, and properties of the product.

Using d-glucurono-6,3-lactone (GlcL) and sucrose (Suc) as raw materials, we synthesized sucuronic acid (SucA), in which the d-glucose (Glc) residue of Suc was replaced with d-glucuronic acid, by a three-step chemoenzymatic method. In the 1st chemical step, methyl d-glucuronate (GlcAM) was synthesized by treating GlcL with a strong base anion exchange resin, Amberlite IRA402BL OH AG, in anhydrous methanol. In the 2nd step, which included an enzyme reaction, methyl sucuronate (SucAM) was synthesized from GlcAM and fructose by exploiting the transfructosylation activity of the Microbacterium saccharophilum K-1 β-fructofuranosidase, a reaction that is suppressed in the presence of high-concentration Glc.

Radiofrequency catheter ablation of a coronary sinus-ventricular accessory connection in dextrocardia with complete situs inversus and an anomalous inferior vena cava.

An anomalous inferior vena cava (IVC) is often associated with patients with dextrocardia. However, radiofrequency catheter ablation in such a case with that combination has not been reported. We encountered a case of Wolff-Parkinson-White syndrome with dextrocardia associated with complete situs inversus and an azygos continuation of the IVC.