Mitochondrial Dynamics of Bcl-2 Family Proteins during 17-β-Estradiol-Induced Apoptosis Correlate with the Malignancy of Endometrial Cancer Cells.

Excessive fat intake leads to an increase in cholesterol. Overexposure to estrogen derived from cholesterol is known to contribute to the malignancy of endometrial adenocarcinomas. However, it is not well understood the relationship between the exposure to estrogen and the malignancy of endometrial adenocarcinomas.

Bone lengthening osteogenesis, a combination of intramembranous and endochondral ossification: an experimental study in sheep.

We evaluated the morphological features of the newly formed tissue in an experimental model of tibial callotasis lengthening on 24 lambs, aged from 2 to 3 months at the time of operation. A unilateral external fixator prototype Monotube Triax(®) (Stryker Howmedica Osteonics, New Jersey) was applied to the left tibia. A percutaneous osteotomy was performed in a minimally traumatic manner using a chisel.

Macrophages that have phagocytosed particles are capable of differentiating into functional osteoclasts.

The aim of the current study was to determine whether human macrophages that have phagocytosed particles are capable of differentiating into osteoclastic bone-resorbing cells. Macrophages isolated from human peripheral blood were cultured with latex particles in the presence of receptor activator of nuclear factor (NF)-kappaB ligand (RANKL) and macrophage colony stimulating factor (M-CSF) on dentine slices and coverslips. After 24 h incubation, particles that had not yet been phagocytosed were removed by washing the slices.

Infliximab acts directly on human osteoclast precursors and enhances osteoclast formation induced by receptor activator of nuclear factor kappaB ligand in vitro.

Infliximab is known to protect against the development of joint destruction. In the present study, we sought to determine whether Infliximab acts directly on human osteoclast precursors and influences monocyte-osteoclast differentiation induced by receptor activator of nuclear factor kappaB ligand (RANKL) in vitro. Peripheral blood mononuclear cells (PBMCs) isolated from rheumatoid arthritis (RA) patients and normal controls were cultured in the presence of RANKL and macrophage colony stimulating factor.

Synovectomy, debridement, and continuous irrigation for infected total knee arthroplasty.

Since 1990, a total of ten joints in nine patients with infected total knee arthroplasty have been treated in our department within 21 days of the onset of infection. Their radiographs showed no evidence of implant loosening or "moth-eaten" appearance. They underwent synovectomy, debridement, and continuous irrigation without implant removal.

Biomechanical study of load transfer of the pubic ramus due to pelvic inclination after hip joint surgery using a three-dimensional finite element model.

We studied load-stress distribution in the pelvis using a three-dimensional finite element model. The results showed that the load-stress on the pubic superior ramus was high in the normal pelvic position without sagittal or coronal inclination following that of the acetabulum in the pelvis. The load-stress on this area was not affected by sagittal pelvic inclination, but it was affected significantly by coronal pelvic inclination.

Radiological and histological analysis of cortical allografts: an experimental study in sheep femora.

Introduction: We developed an experimental model in sheep femora to evaluate the process of cortical allograft incorporation.

Materials And Methods: Twenty-four sheep were divided into four groups according to the various treatments of cortical allografts as follows: fresh, frozen, autoclaved, and frozen with perforation. Periodical radiographic and histological evaluations were performed for each group.

Limb salvage for malignant bone and soft-tissue tumours of the shoulder girdle.

We treated 15 patients with malignant bone and soft-tissue tumours of the shoulder girdle using limb salvage operations involving resection of the proximal humerus, scapula and clavicle. The oncological and functional outcomes were evaluated in all patients after an average of 5 (1-13) years. Oncological evaluation revealed that in 11 patients with adequate surgical margins six were continuously disease free and two had no evidence of disease.

Carpal tunnel syndrome and trigger wrist caused by a lipoma arising from flexor tenosynovium: a case report.

We report on a patient with lipoma arising from flexor tenosynovium at the level of the wrist who had snapping of the middle finger and carpal tunnel syndrome. She was relieved of these symptoms after excising the lipoma.

Influence of aeration, storage, and rinsing conditions on residual ethylene oxide in freeze-dried bone allograft.

Bone allografts sterilized with ethylene oxide gas (EO) are used in the field of orthopedic surgery, and the reduction of the EO residual concentration is an urgent clinical matter. We therefore investigated the efficacy of aeration and the effects of varied preservation periods and rinsing conditions on the reduction of EO residuals in freeze-dried bone allografts in the present study. Before aeration, the EO residual level was 12.