Dental age estimation based on DNA methylation using real-time methylation-specific PCR.

Age estimation is crucial for reconstructing the biological profiles of deceased victims in the forensic field. DNA methylation, which varies in an age-dependent manner in specific genes, is a candidate biomarker for estimating chronological age. DNA methylation-based models for estimating age have been developed using various technologies such as pyrosequencing.

Effect of cranium structure on dose distribution during intraoral radiography.

Objectives: This study aimed to evaluate, the effect of the cranium structure on dose distribution by measuring the dose using the RANDO phantom for safe use of an X-ray unit during personal identification work at a large-scale disaster site.

Methods: As the subject, the head and neck of the RANDO Phantom (Alderson) containing a dry skull composed of a tissue-equivalent substance and having a shape similar to that of a living body were studied. With the body in the supine position, the air doses per second of irradiation time were measured for a total of six sections of the anterior, premolar, and molar teeth (molar on the left side) at a tube voltage of 60 kV and tube current of 10 mA during maxilla and mandible intraoral radiography.

A newly developed age estimation method based on CpG methylation of teeth-derived DNA using real-time methylation-specific PCR.

Age estimation of unidentified bodies is important in forensic medicine and crime scenes. There is accumulating evidence that DNA methylation in the human genome isolated from body fluids changes with age. Most of the data have been obtained by pyrosequencing.

Comparison of air dose and operator exposure from portable X-ray units.

This study was aimed at investigating and comparing exposure dose of workers and the surrounding workers. In addition, worker's exposure was also measure about lens and finger. Four intraoral portable X-ray units were evaluated.

Age estimation based on the volume change in the maxillary premolar crown using micro CT.

Age is often estimated using teeth because numerous external and internal changes appear due to aging. The purpose of this study was to investigate an age estimation method based on the volume ratio of the internal structure of the crown. 61 maxillary first premolars and 50 maxillary second premolars from subjects aged 20-79 years old were used.

Assessment of quality and interpretation of panoramic radiographs obtained in the Lao People's Democratic Republic as part of a teleradiology collaboration with Japan.

As part of quality assessment of a teleradiology program we evaluated the validity of patient information received, the quality of panoramic radiography imaging in Laos, and the ability of a Laotian radiologist to detect temporomandibular joint abnormalities. The amount of patient information gathered from 2,021 scans of panoramic radiographs was evaluated by triage before image diagnosis. Among the radiographs from 2,021 patients, primary triage indicated that there was insufficient information for 794 (39.

The oral health of children in a rural area of the Lao People's Democratic Republic.

The lack of information on oral health in Laos makes it difficult to estimate the need and methods for preventing oral disease. This study identified problems concerning the oral health of Lao children. The study subjects were 59 school children who lived in Pakkading District.

EDX fluorescence analysis and SEM observations of resin composites.

The purpose of this study was to evaluate the filler compositions of recently available light-cured resins. The composition of each resin paste was evaluated using an energy dispersive X-ray fluorescence spectrometer. Scanning electron microscopic observation of the polymerized resin pastes was also conducted.

Comparative study of craniofacial morphology and bite force in Fijians and Japanese.

An anthropological survey was conducted in Fiji in 1994 and 1995 to study dental arch form, craniofacial morphology, and bite force of Fijians. Measurements were obtained from dental casts, cephalograms, and thin pressure-sensitive sheets (Dental Prescale®) for bite force analysis. Results were compared with those of Japanese.