Publications by authors named "Hirah Amir"
The aim of this study was to purify BMP-2 in an easy and time-efficient way. We have developed a new method in which BMP-2 is produced through leaky expression in E. coli BL21 (DE3) cells as inclusion bodies, eliminating the need for inducer Isopropyl β-D-1-thiogalactopyranoside (IPTG).
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- The study explores the use of subcritical water hydrolysis to extract proteins and bioactive compounds from the macroalga Padina tetrastromatica, highlighting its potential as a sustainable source of amino acids and peptides.
- The optimal extraction conditions identified were 220 °C for 10 minutes, resulting in the recovery of 79% protein and a significant amount of essential amino acids, with an emphasis on methionine, isoleucine, and leucine.
- Additionally, the hydrolysate demonstrated antioxidant properties and showed potential in inhibiting enzymes linked to Alzheimer's disease and diabetes, suggesting its applications in nutraceutical, pharmaceutical, and cosmeceutical fields.