Publications by authors named "Hira Kamal"

This review article delves into the vital aspects of cotton, emphasizing its global significance as a crucial agricultural commodity. The paper comprehensively explores the composition of cotton and surveys the diverse methods employed for the removal of cotton lint from seeds. Conventional delinting methods, including mechanical and chemical approaches, are scrutinized in terms of their advantages and drawbacks.

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Potato mop-top virus (PMTV) is an emerging viral pathogen that causes tuber necrosis in potatoes. PMTV is composed of three single-stranded RNA segments: RNA1 encodes RNA-dependent RNA polymerase, RNA2 contains the coat protein (CP), and RNA3 harbors a triple gene block (TGB 1, TGB2, and TGB3). CP plays a role in viral transmission, while TGB is known to facilitate cell-to-cell and long-distance systemic movement.

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During plant-pathogen interaction, plant exhibits a strong defense system utilizing diverse groups of proteins to suppress the infection and subsequent establishment of the pathogen. However, in response, pathogens trigger an anti-silencing mechanism to overcome the host defense machinery. Among plant viruses, geminiviruses are the second largest virus family with a worldwide distribution and continue to be production constraints to food, feed, and fiber crops.

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Plant viruses are the most abundant destructive agents that exist in every ecosystem, causing severe diseases in multiple crops worldwide. Currently, a major gap is present in computational biology determining plant viruses interaction with its host. We lay out a strategy to extract virus-host protein interactions using various protein binding and interface methods for Geminiviridae, a second largest virus family.

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Article Synopsis
  • The study investigated the population structure and genetic diversity of 100 barley lines over three years in Pakistan and China, revealing four main subpopulations and a high level of genetic variation.
  • The analysis identified 133 alleles and calculated average gene diversity, supporting the classification of lines into four phylogenetic groups based on SSR marker data.
  • The research culminated in the creation of a core set of 20 barley genotypes, highlighting specific SSR markers for traits like malt quality and salinity tolerance, which can enhance marker-assisted breeding.
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Powdery scab disease, caused by the soilborne protist f. sp. , poses a major constraint to potato production worldwide.

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Potato virus Y (PVY) is an economically important plant pathogen that reduces the productivity of several host plants. To develop PVY-resistant cultivars, it is essential to identify the plant-PVY interactome and decipher the biological significance of those molecular interactions. We performed a yeast two-hybrid (Y2H) screen of cDNA library using PVY-encoded NIa-pro as the bait.

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Begomoviruses interfere with host plant machinery to evade host defense mechanism by interacting with plant proteins. In the old world, this group of viruses are usually associated with betasatellite that induces severe disease symptoms by encoding a protein, βC1, which is a pathogenicity determinant. Here, we show that βC1 encoded by Cotton leaf curl Multan betasatellite (CLCuMB) requires Gossypium hirsutum calmodulin-like protein 11 (Gh-CML11) to infect cotton.

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Cotton leaf curl disease (CLCuD) caused by viruses of genus is a major constraint to cotton () production in many cotton-growing regions of the world. Symptoms of the disease are caused by Cotton leaf curl Multan betasatellite (CLCuMB) that encodes a pathogenicity determinant protein, βC1. Here, we report the identification of interacting regions in βC1 protein by using computational approaches including sequence recognition, and binding site and interface prediction methods.

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Article Synopsis
  • A field survey revealed that symptomatic spinach leaves contained a virus called chickpea chlorotic dwarf virus (CpCDV), linked to visible leaf issues like curling and darkening.
  • Researchers used techniques like rolling circle amplification (RCA) and sequencing to confirm the presence of CpCDV and its association with two types of satellite viruses (CLCuMB and CLCuMA).
  • This study is noteworthy as it reports, for the first time, the infection of spinach (a dicot plant) by CpCDV and its satellite viruses in Pakistan, contributing to our understanding of plant virus interactions and diseases.
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A Tobacco rattle virus (TRV)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (Gossypium hirsutum). This approach was used to investigate the function of the Enoyl-CoA reductase (GhECR) gene in pathogen defense. Amino acid sequence alignment of Arabidopsis ECR with homologous sequence from G.

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