Increasing Quality and Frequency of Goals-of-Care Documentation in the Highest-Risk Surgical Candidates: One-Year Results of the Surgical Pause Program.

Unlabelled: Patient values may be obscured when decisions are made under the circumstances of constrained time and limited counseling. The objective of this study was to determine if a multidisciplinary review aimed at ensuring goal-concordant treatment and perioperative risk assessment in high-risk orthopaedic trauma patients would increase the quality and frequency of goals-of-care documentation without increasing the rate of adverse events.

Methods: We prospectively analyzed a longitudinal cohort of adult patients treated for traumatic orthopaedic injuries that were neither life- nor limb-threatening between January 1, 2020, and July 1, 2021.

Mouse beta-defensin-14, an antimicrobial ortholog of human beta-defensin-3.

Searching the database for mouse homologs of the antimicrobial peptide human beta-defensin-3 (hBD-3) revealed highest identity (69%) to mouse beta-defensin-14 (mBD-14). Recombinant mBD-14 exhibited broad-spectrum, nanomolar microbicidal activity. Treatment of keratinocytes with gamma interferon or transforming growth factor alpha increased mBD-14 gene expression.

Principles of ontogenesis of leg and foot in man.

Human leg and foot anlagen of different developmental stages were studied by means of light and scanning electron microscopy. The findings were compared with principles of human arm and hand development and results obtained experimentally from chicken limbs. The limbs studied have in common the shaping, cell differentiation, and spatial arrangement of different cells as basic processes of development.

The development of cellular cementum in rat molars, with special reference to the fiber arrangement.

To elucidate how intrinsic and extrinsic fibers are arranged during cementogenesis, rat cellular cementum was observed in various developmental stages by light and electron microscopy. As cementogenesis progressed, the periodontal ligament cells showed characteristics of collagen-secreting cells, wing-like processes, and delimited extracellular compartments. The principal fibers were organized into substantial bundles in these compartments.

[Development of the definite angle of the mouth in man].

Until today different views exist on the development of the cheek and the final angle of the mouth. Recently, new results have been found indicating a cialisation of the epithelium during the fusion or adhesion of prominences covered by epithelia. Our results led to further information on the development of the angle of the mouth.

The onset of myotome formation in the chick.

The onset of myotome formation in somites of chick embryos was studied by use of a polyclonal antidesmin antibody and by histochemical demonstration of acetylcholine esterase activity. The myotome cells originate from the dermatome only; sclerotome cells do not contribute to the myotome. The formation of the myotome starts in the craniomedial corner of the dermatome.

Morphological aspects of the pharyngeal hypophysis in human embryos.

In human embryos the hypophyseal sac (Rathke's pouch) originates at the roof of the mouth until stage 15 as a broad rim. As the mandibular arch and the maxillary swelling enhance the mesodermal masses in forming the early palatal shelves the rim is reduced to a cleft of about 0.2 mm in broadness in stage 17.

Ultrastructure of the ameloblast-stratum intermedium border during ameloblast differentiation.

The molar tooth germs of newborn mice were isolated and the zone of ameloblast differentiation studied with the electron microscope. Special attention was paid to the changes that occur in the contacts such as desmosomes, tight junctions and close attachments associated with cell interdigitations between the inner enamel epithelium (IEE) and the stratum intermedium (SI) cells from the beginning of differentiation to the onset of enamel secretion. These changes are accompanied by variations in the width and configuration of the intercellular space separating the two cell layers.

Semiautomated evaluation of morphometric data on myoblasts and myotubes and the calculation of the relative DNA content with the television texture analysis system (TAS).

In cell cultures of skeletal muscles from 11 day-old chick embryos, myoblasts begin to fuse to myotubes on the second day of culture. Morphological data such as area of projection of the nucleus, length of myotubes and number of nuclei per myotube, as well as histochemical data such as relative DNA content and degree of chromatin condensation were evaluated. The myotubes examined, up to 216 h in culture, had an average of 7 nuclei.

Principles and methods for the quantitative determination of Feulegen stained DNA with the television texture analysis system (TAS).

The television texture analysis system (TAS, Leitz) has been applied to determination of the relative DNA content of Feulgen stained nuclei. The principles of the method are described and comparative measurements are presented. The determination is based on the measurement of areas at preset intervals of transmittance, taking into consideration the necessary correction of extinction values in relation to area and applying a correction for background.

[The histogenesis of somites in the chick].

The somites of the thoracic region of chick embryos incubated for 2 (stages 13-15) and 3 days (stages 17-19) have been examined cytologically and ultrastructurally. Cell contacts and cell arrangement were studied first in spherical somites. The further development of the spherical somite is characterized by changes in the cell arrangement.

Melanogenesis in the pigment epithelium of chicken embryos. I. Topogenesis of pigment in the iris anlage.

The iris anlage of 2-10 and 15 days old chicken embryos were studied histochemically, and by both light and electron microscopy. Light microscopic serial sections showed that pigmentation began at the outer layer of the posterior eye pole and progressed from there forwards to the optic cup margin. The entire outer layer of the optic cup as well as the pupillary margin were completely pigmented by the 4th day of incubation.