Expression of the Arrowhead Proteinase Inhibitor B (API) Gene in the Silkworm Larvae and Pupae.

The arrowhead proteinase inhibitor B (API) gene was cloned into baculovirus transfer vectors of pBacPAK8 and pOSX, and two recombinant transfer vectors, pBacPAK (API) and pOSX (API), were constructed. Co-infection was accomplished with either one of the recombinant transfer vectors and Bm-BacPAK6 DNA in BmN cells. Then recombinant virus of CrBK9, CrBK10 and BX was selected.