Novel enhanced detection and resolution of a nonfluorescent mixture in plasma at nanogram levels using sustainable fluorescent carbon dots and advanced chemometric models.

For the first time, advanced chemometric models were utilized to determine florescence induced by carbon dots. In an endeavor to regulate anthelmintic drug usage by facilitating the determination of veterinary formulations in animals' biological fluids, a novel fluorometric-assisted chemometric method has been developed for detecting two nonfluorescent drugs, Ivermectin (IVR) and Clorsulon (CLR). The method relies on the linear quenching effect of the drugs on the fluorescence intensity of carbon dots (CDs) synthesized from natural sources.

Eco-friendly electrochemical assay of oxytetracycline and flunixin in their veterinary injections and spiked milk samples.

Two solid-contact electrochemical sensors were developed for detection of each of oxytetracycline HCl (OXY), and the co-formulated non-steroidal anti-inflammatory drug flunixin meglumine (FLU) in veterinary formulations and animal-derived food products. The designed sensors were based on a glassy carbon electrode as the substrate material and high molecular weight polyvinyl chloride (PVC) polymeric ion-sensing membranes doped with multiwalled carbon nanotubes (MWCNTs) to improve the potential stability and minimize signal drift. For determination of OXY, the sensing membrane was modified with potassium tetrakis (4-chlorophenyl) borate (K-TCPB), which was employed as a cation exchanger, and 2-hydroxypropyl-β-cyclodextrin (HP-ßCD), which was used as an ionophore.

Utilizing two different sustainable and green spectrophotometric approaches using derivative ratio spectra for the determination of a ternary severely overlapped mixture: application to veterinary formulation.

Mathematical manipulation technique has proven to be a very powerful tool for efficient processing and handling of highly overlapped spectra. This work introduced two green and sustainable approaches for the successful recovery of the ternary mixture, Tylosin tartarate (TYL), Sulfadimdine (SLD), and Trimethoprim (TRI). The approaches were constructed using three different methods, derivative ratio spectrum zero-crossing method (DRSZ), double divisor ratio spectra derivative method (DDRD), and factorized derivative ratio method coupled with spectrum subtraction (FDRM-SS).

Smart Eco-Friendly Spectrophotometric Methods Resolving Highly Overlapping Spectra: Application to Veterinary Antibiotic Injections.

Background: Over the last few years, mathematical manipulation has proved to be a very powerful means of successfully resolving severely overlapped spectra for various multicomponent mixtures.

Objective: Smart and environmentally friendly spectrophotometric determination approaches were used for two binary mixtures of fixed dose veterinary injections containing flunixin meglumine (FLU) combined with either florfenicol (FLR), or oxytetracycline HCl (OXY).

Methods: Regarding the first mixture, both FLU and FLR were determined by three successive resolution techniques, which were; constant multiplication coupled with spectrum subtraction (CM-SS), derivative ratio (DD1), and ratio difference (RD), and two progressive resolution techniques which were absorbance subtraction (AS) and amplitude modulation (AM).