Serotonin- and eicosanoid-dependent rapid hemocyte aggregation in the hemolymph is the first step in nodule formation in Bombyx mori larvae.

Nodule formation is a well-known process in cellular immunity of insects. However, few studies have investigated the role of hemocytes in rapid aggregation before tissue adhesion. In addition, since nodule formation is usually elicited by injecting large quantities of microbes, it remains unclear whether nodule formation is a natural response.

Nodule formation in Bombyx mori larvae is regulated by BmToll10-3.

Nodule formation is a two-step cell-mediated immune response that is elicited by the cytokine spätzle1. Spätzle1 is activated within 30 s of invasion by microorganisms via an extracellular signaling pathway that consists of pathogen-associated molecular pattern recognition receptors, C-type lectins, and serine proteases. Here, we investigated a hemocyte molecule that is involved in eliciting the first step of nodule formation.