Endogenous CCL21-Ser deficiency reduces B16-F10 melanoma growth by enhanced antitumor immunity.

The chemokine CCL21 regulates immune and cancer cell migration through its receptor CCR7. The gene encodes the isoform CCL21-Ser, predominantly expressed in the thymic medulla and the secondary lymphoid tissues. This study examined the roles of CCL21-Ser in the antitumor immune response in -knockout (KO) mice.

Firefly luciferase-based chronological measurement of effector CD8 T-cell activity using a multi-chamber luminometer.

Although cell-mediated cytotoxicity has been evaluated with various protocols, methods for monitoring cytotoxicity in a time series have not been established. This work describes a method for evaluating cytotoxicity using a multi-chamber real-time luminometer. The efficiency of effector CD8 T-cell expansion from melanoma-bearing splenocytes was analyzed.