Estimation, Evaluation and Characterization of Carbapenem Resistance Burden from a Tertiary Care Hospital, Pakistan.

Carbapenem resistance has become major concern in healthcare settings globally; therefore, its monitoring is crucial for intervention efforts to halt resistance spread. During May 2019-April 2022, 2170 clinical strains were characterized for antimicrobial susceptibility, resistance genes, replicon and sequence types. Overall, 42.

Emergence of multidrug-resistant ST11 blaKPC-2 producing Klebsiella pneumoniae coharboring blaCTX-M and blaSHV in Pakistan.

Introduction: Carbapenemases are primarily responsible for the intensified spread of multidrug-resistant (MDR) K. pneumoniae by virtue of antibiotics overuse. Therefore, frequent investigation of high-risk clones especially from developing world is crucial to curtail global spread.

Characterization of Genomic Diversity among Carbapenem-Resistant Clinical Isolates and Antibacterial Efficacy of Silver Nanoparticles from Pakistan.

The emergence of carbapenem-resistant () is considered an important threat to public health resulting in resistance accumulation due to antibiotics misuse and selection pressure. This warrants periodic efforts to investigate and develop strategies for infection control. A total of 184 carbapenem-resistant clinical strains of were characterized for resistance pattern, resistance genes, plasmids, sequence types and in vitro efficacy of silver nanoparticles (AgNPs).

Impact of antibiotic withdrawal and starvation conditions on plasmid elimination and consequent loss of resistance.

Objective: To assess resistance-loss due to plasmid elimination under experimental conditions, including withdrawal of antibiotics and administration of starvation conditions.

Methods: The experimental study was conducted at the Department of Pathology, King Edward Medical University, Lahore, Pakistan, from July to December 2019. A single sensitive clinical isolate of escherichia coli, showing resistance towards ampicillin was collected and separately sub-cultured in three different culture broths: tryptic soya broth, minimal broth and control broth for a period of one month under standard laboratory conditions.

A rare case of extensively drug-resistant typhi meningitis in a 9-year old: a case report.

Meningitis caused by typhi is rare and is generally seen in infants. We report a case of a 9-year-old boy with extensively drug-resistant typhi meningitis. The patient was diagnosed using a culture sensitivity test and successfully treated with meropenem.

Three years mortality analysis in general surgery patients.

Objective: Surgical patient mortality is progressively being measured for providing better management and care in different healthcare systems world-wide. The aim of this study was to assess mortality within a surgical department and to evaluate components associated with surgical and non- surgical deaths.

Methods: We retrospectively collected data including all admissions, both operative and non-operative, surgical procedures and reason of admission (for non-operative) and mortalities within three-year period (2015-2017) from Surgical Unit-2, JPMC Karachi.