The enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of schistosomiasis.

The enzyme-linked immunosorbent assay (ELISA) done with soluble egg antigens (SEA) of Schistosoma mansoni was utilized for the detection of infections with schistosomes. The method readily detected experimental infections in NIH outbred, New Zealand black, and New Zealand white mice by 6-10 weeks post-exposure to S. mansoni cercariae.

Solubilization of antigens of Fasciola hepatica which react with antibodies to Schistosoma mansoni.

Extracts of Fasciola hepatica adult worms contain antigens reactive with antisera prepared against Schistosoma mansoni adult worms. These antigens are poorly solubilized when homogenized in a phosphate buffered saline (PBS) solution and pellet readily when subjected to high speed centrifugation. Solubilization is improved greatly by the addition of sodium dodecyl sulfate (0.

Demonstration of a common antigen between Schistosoma mansoni and Fasciola hepatica.

The presence of a common antigen between Schistosoma mansoni eggs and Fasciola hepatica adult worms was demonstrated by utilizing in an anti-S. mansoni adult worm antiserum. Although not one of the three major serologic S.

Laurell crossed immunoelectrophoresis and affinity chromatography for the purification of a parasite antigen.

Laurell crossed immunoelectrophoresis (two-dimensional electroimmunodiffusion) was used to prepare minute amounts of purified parasite antigens complexed with their precipitating antibodies obtained from rabbits. These complexes, emuslified in Freund's complete adjuvant, were then used to prime rabbits for selective production of precipitins to the complexed antigens when the animals were later boosted with whole parasite extract. The IgG antibody from the monospecific antiserum recovered was then utilized in affinity chromatography to isolate from the crude parasite antigen large amounts of specific antigen in one step.

Partial purification of fasciola hepatica antigen for the immunodiagnosis of fascioliasis in rats.

When F. hepatica adult worm antigen is fractionated with Sephadex G-200, four major peaks are obtained. The first peak, when used as antigen in counterelectrophoresis, reacts with the serum of normal rats as well as from rats infected with F.

Schistosome antigens in the circulation of chimpanzees infected with Schistosoma japonicum.

Chimpanzees infected with Schistosoma japonicum develop circulating schistosome antigens in their circulation between 6 and 9 weeks post-exposure. The minimum number of circulating antigens ranges from one to three. These antigens also cross-react with an antiserum against S.

Use of immunologic techniques to detect chemotherapeutic success in infections with Fasciola hepatica. I. Rabbit infections.

Rabbits infected with Fasciola hepatica develop precipitins to adult worm heomogenates, as observed by Ouchterlony double immunodiffusion (ID) and counterelectrophoresis (CEP). When they are successfully treated with a fasciolicidal drug such as rafoxanide at 5, 6, or 11 weeks of infection their precipitins drop dramatically by 2 weeks post-treatment, they are virtually negative by 4 weeks, and have no detectable precipitins by 5 or 6 weeks post-treatment. The results suggest that ID or CEP can be utilized to show chemotherapeutic success in rabbits infected with F.