Structure and activation mechanism of the yeast RNA Pol II CTD kinase CTDK-1 complex.

The C-terminal domain (CTD) kinase I (CTDK-1) complex is the primary RNA Polymerase II (Pol II) CTD Ser2 kinase in budding yeast. CTDK-1 consists of a cyclin-dependent kinase (CDK) Ctk1, a cyclin Ctk2, and a unique subunit Ctk3 required for CTDK-1 activity. Here, we present a crystal structure of CTDK-1 at 1.

Maintaining Sense of Purpose Following Health Adversity in Older Adulthood: A Propensity Score Matching Examination.

Objectives: Research has demonstrated sense of purpose predicts better health in older adulthood. However, work is limited with respect to understanding how experiencing a health event or illness diagnosis impacts older adults' sense of purpose.

Method: The current study employed a propensity score matching approach to compare older adults who did or did not experience an adverse health event on changes in sense of purpose across 3 waves of the Health and Retirement Study.

Global health security and universal health coverage: Understanding convergences and divergences for a synergistic response.

Background: Global health security (GHS) and universal health coverage (UHC) are key global health agendas which aspire for a healthier and safer world. However, there are tensions between GHS and UHC strategy and implementation. The objective of this study was to assess the relationship between GHS and UHC using two recent quantitative indices.

Associations between depressive symptoms with perceived future time and opportunities: tests of unique prediction and moderation.

Objectives: Having a broadened perspective on one's future has been associated with better affective well-being, including reduced reports of depressive symptoms. However, research is limited regarding which aspect of future time perspective is associated with depressive symptoms, and whether these findings are consistent across individuals.

Methods: The current study employed data from a nationally representative sample of Swiss adults ( = 1774; mean age: 49.

Subungal squamous cell carcinoma of the thumb - Implications for patient setup and radiotherapy planning.

Subungal squamous cell carcinoma (SSCC) is a rare malignancy of the nail bed. We present the case of a woman diagnosed with a SSCC of the right thumb. Initial management consisted of surgically excision followed by adjuvant radiotherapy due to a positive bone margin.

The Effect of Kidney Biopsy on Glomerular Filtration Rate: A Frequent Patient Concern.

The effect of percutaneous kidney biopsy on glomerular filtration rate has never been identified, though it is frequently a concern raised by patients. Following a clinical interaction with an inquisitive patient undergoing her fifth biopsy, we attempted to estimate the effect using retrospective data. In a cohort of patients with stable kidney function undergoing transplant biopsy without clinical indication (as part of a surveillance programme) the effect of biopsy was observed as a step change in glomerular filtration rate.

Primary health care contributions to universal health coverage, Ethiopia.

Many global health institutions, including the World Health Organization, consider primary health care as the path towards achieving universal health coverage (UHC). However, there remain concerns about the feasibility and effectiveness of this approach in low-resource countries. Ethiopia has been implementing the primary health-care approach since the mid-1970s, with primary health care at the core of the health system since 1993.

Neuroticism predicts informant reported cognitive problems through health behaviors.

Objective: Personality traits have been linked to cognitive impairment, though work is needed to understand the mechanisms involved. Research also needs to consider alternative markers of cognitive impairment, such as informant report measures. The aim of the current study was to examine the role of health behaviors and social engagement as mediators for the relationship between personality and informant reported cognitive problems.

Body Reprogramming: Reframing the Fibromyalgia narrative and providing an integrative therapeutic model.

We propose a theory known as the Hyland model to help conceptualise Fibromyalgia within a complex adaptive control system. A fundamental assumption is that symptom generating mechanisms are causally connected, forming a network that has emergent properties. An illness narrative has been developed which has a 'goodness of fit' with the lived experience of those with Fibromyalgia.

Moving beyond promoting 'Happiness' in gerontology interventions.

Researchers have called for gerontologists to spend greater attention on promoting happiness in older adulthood, a point aligned with the general public's interest in finding the keys to being happy later in life. However, targeting and even defining happiness comes with several caveats and challenges, leaving researchers to make difficult decisions regarding measurement and intervention strategies. Instead, the current commentary suggests that gerontology interventions may fare better if researchers focus on specific components of positive psychological functioning.

A public health approach to increase treatment of latent TB among household contacts in Brazil.

Two consecutive trials were conducted to evaluate the effectiveness of a public health approach to identify and correct problems in the care cascade for household contacts (HHCs) of TB patients in three Brazilian high TB incidence cities. In the first trial, 12 clinics underwent standardised evaluation using questionnaires administered to TB patients, HHCs and healthcare workers, and analysis of the cascade of latent TB care among HHCs. Six clinics were then randomised to receive interventions to strengthen management of latent TB infection (LTBI), including in-service training provided by nurses, work process organisation and additional clinic-specific solutions.

Video chatting and appearance satisfaction during COVID-19: Appearance comparisons and self-objectification as moderators.

Objective: As video chatting has emerged as a leading form of communication for work, education, and socialization during the COVID-19 pandemic, it is important to investigate the association between video chatting and appearance satisfaction.

Method: Participants included women from the United States (n = 438; age: M = 31.3, SD = 12.

Clean manufacturing powered by biology: how Amyris has deployed technology and aims to do it better.

Amyris is a fermentation product company that leverages synthetic biology and has been bringing novel fermentation products to the market since 2009. Driven by breakthroughs in genome editing, strain construction and testing, analytics, automation, data science, and process development, Amyris has commercialized nine separate fermentation products over the last decade. This has been accomplished by partnering with the teams at 17 different manufacturing sites around the world.

Mapping the Ultrasound Landscape to Define Point-of-Care Ultrasound and Diagnostic Ultrasound: A Proposal From the Society of Radiologists in Ultrasound and ACR Commission on Ultrasound.

Current descriptions of ultrasound evaluations, including use of the term "point-of-care ultrasound" (POCUS), are imprecise because they are predicated on distinctions based on the device used to obtain images, the location where the images were obtained, the provider who obtained the images, or the focus of the examination. This is confusing because it does not account for more meaningful distinctions based on the setting, comprehensiveness, and completeness of the evaluation. In this article, the Society of Radiologists in Ultrasound and the members of the American College of Radiology Ultrasound Commission articulate a map of the ultrasound landscape that divides sonographic evaluations into four distinct categories on the basis of setting, comprehensiveness, and completeness.

Operational Recommendations for Scarce Resource Allocation in a Public Health Crisis.

The coronavirus disease 2019 pandemic may require rationing of various medical resources if demand exceeds supply. Theoretical frameworks for resource allocation have provided much needed ethical guidance, but hospitals still need to address objective practicalities and legal vetting to operationalize scarce resource allocation schemata. To develop operational scarce resource allocation processes for public health catastrophes, including the coronavirus disease 2019 pandemic, five health systems in Maryland formed a consortium-with diverse expertise and representation-representing more than half of all hospitals in the state.

Patient Trajectories Among Persons Hospitalized for COVID-19 : A Cohort Study.

Background: Risk factors for progression of coronavirus disease 2019 (COVID-19) to severe disease or death are underexplored in U.S. cohorts.

Cross-lagged relationships between sense of purpose in life, memory performance, and subjective memory beliefs in adulthood over a 9-year interval.

Objectives: Previous studies have found a positive association between having a sense of purpose in life and memory functioning in old age. We extend these findings by examining the relationships between sense of purpose, memory performance, and subjective memory beliefs over time in a large sample of adults in mid to later adulthood.

Method: We used data from 3633 participants of the second and third wave of the MIDUS study.

The Relationship Between Career Success and Sense of Purpose: Examining Linkages and Changes.

Objectives: Although researchers have linked sense of purpose to working status, there are few studies examining how specific characteristics of work may correlate with sense of purpose. The aim of the current study is to extend prior research by assessing the degree to which objective and subjective forms of career success-occupational prestige and work satisfaction-are associated with levels of and changes in sense of purpose.

Methods: Participants were part of the Rochester Adult Longitudinal Study (N = 307), which contains multiple cohorts of participants each assessed at multiple waves (full age range: 42-71).

Age Differences In Retrieval-Related Reinstatement Reflect Age-Related Dedifferentiation At Encoding.

Age-related reductions in neural selectivity have been linked to cognitive decline. We examined whether age differences in the strength of retrieval-related cortical reinstatement could be explained by analogous differences in neural selectivity at encoding, and whether reinstatement was associated with memory performance in an age-dependent or an age-independent manner. Young and older adults underwent fMRI as they encoded words paired with images of faces or scenes.

Does being active mean being purposeful in older adulthood? Examining the moderating role of retirement.

The degree to which individuals engage in leisure activities has been shown to predict well-being in older adults, but it is not known whether such activities may help older adults maintain purposefulness into retirement. The current study sought to address whether activity engagement is associated with purpose in life and whether this association differs based on retirement status. We used data from three waves of the Health and Retirement Study between 2008 and 2016.