Effect of antioxidant supplementation on leucocyte expression of reactive oxygen species in athletes.

Objective: Previous studies have shown that leucocytes, in particular granulocytes, have an enormous capacity for reactive oxygen species (ROS) production, and that this can be influenced by the physical activity of the individual. Theoretically, endurance-trained athletes could profit by increasing their intake of antioxidants, thus neutralizing increased ROS production. The aim of the present investigation was to study the effect of dietary antioxidant supplementation on leucocyte ROS expression and total plasma antioxidant status (TAS) in endurance-trained athletes over the course of 4 weeks.

LeuCAM and reactive oxygen species during long-term exercise combined with sleep and energy deficiency.

Introduction: During the Norwegian military ranger-training course, cadets are exposed to prolonged physical exercise combined with sleep-, energy-, and food deficiency. The open-window postexercise hypothesis indicates that after hard physical activity, there is an increased risk of contracting infectious diseases.

Purpose: The purpose of the present study was to determine leukocyte reactive oxygen species (ROS) levels, total antioxidant status (TAS), leukocyte expression of the cell adhesion molecules CD62L and CD11b, and plasma levels of soluble adhesion molecule L-selectin before, during, and in the recovery phase of a military ranger-training course.

Marathon running leads to partial exhaustion of ROS-generating capacity in leukocytes.

Purpose: The aim of the present study was to investigate the changes occurring in leukocyte levels of reactive oxygen species (ROS) and total blood plasma antioxidant capacity (TAS) as a result of a marathon/half-marathon race.

Methods: Fourteen men participating in the Oslo Marathon 2000 and 8 women and 8 men participating in the Oslo Half-Marathon 2001 were recruited to the study. Flow cytometry and the ROS-sensitive probe dihydroethidium (DHE) were used to study the intracellular levels of ROS in circulating leukocytes.