Publications by authors named "Hilal Gurler"
The goals of this study were to optimize the medium formulation for enhanced production of Aspergillus niger inulinase using Plackett-Burman Design (PBD) and to model the fermentation in optimal medium formulation. Results indicated that (NH)SO (negative effect), yeast extract and peptone (positive effect) were determined as significant factors affecting the inulinase production. Different media including Medium A (non-enriched), Medium B (contains both negative and positive factors) and Medium C (contains only positive factors) were formed and inulinase fermentations were performed.
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- * A study investigated the expression of the fractalkine receptor (CX3CR1) and its ligand (CX3CL1) to compare gene expression patterns in fallopian adenocarcinoma and high-grade serous ovarian carcinoma.
- * The results showed CX3CR1 present in both normal and cancerous fallopian epithelium, while CX3CL1 was mostly absent in malignant samples; indicating that these cancers, though linked, may have distinct molecular features.
Objective: Genomic studies of ovarian cancer (OC) cell lines frequently used in research revealed that these cells do not fully represent high-grade serous ovarian cancer (HGSOC), the most common OC histologic type. However, OC lines that appear to genomically resemble HGSOC have not been extensively used and their growth characteristics in murine xenografts are essentially unknown.
Methods: To better understand growth patterns and characteristics of HGSOC cell lines in vivo, CAOV3, COV362, KURAMOCHI, NIH-OVCAR3, OVCAR4, OVCAR5, OVCAR8, OVSAHO, OVKATE, SNU119 and UWB1.
Epithelial ovarian carcinoma is the deadliest gynecologic malignancy. One reason underlying treatment failure is resistance to paclitaxel. Expression of the microtubule associated protein tau has recently been proposed as a predictor of response to paclitaxel in ovarian carcinoma patients.
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- Epithelial ovarian carcinoma (EOC) is a severe cancer that often leads to metastasis in the peritoneal cavity, with the role of cell aggregates, or spheroids, being significant in this process.
- The study investigated how versican, a proteoglycan, affects the adhesion of EOC spheroids and cells to peritoneal tissue, using both in vitro cell cultures and in vivo mouse models.
- Results showed that lower versican levels hindered spheroid adhesion and migration, leading to a reduced capacity for tumor formation in the peritoneum, indicating that versican is crucial for metastasis development in EOC.
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- * Researchers used immunohistochemistry on ovarian teratoma and human fetal tissue samples, finding that most specimens contained CX3CR1-positive cells primarily from an epidermal lineage.
- * Results showed that downregulating CX3CR1 in ovarian teratocarcinoma cells prevented the expression of a keratinocyte marker, indicating CX3CR1's crucial role in the differentiation process of epidermal cells.
Cells respond to changes in the physical properties of the extracellular matrix with altered behavior and gene expression, highlighting the important role of the microenvironment in the regulation of cell function. In the current study, culture of epithelial ovarian cancer cells on three-dimensional collagen I gels led to a dramatic down-regulation of the Wnt signaling inhibitor dickkopf-1 with a concomitant increase in nuclear β-catenin and enhanced β-catenin/Tcf/Lef transcriptional activity. Increased three-dimensional collagen gel invasion was accompanied by transcriptional up-regulation of the membrane-tethered collagenase membrane type 1 matrix metalloproteinase, and an inverse relationship between dickkopf-1 and membrane type 1 matrix metalloproteinase was observed in human epithelial ovarian cancer specimens.
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