This study developed multiplex real-time PCR methods using SYBR Green and TaqMan probes to quickly and accurately diagnose three types of pathogenic Yersinia: highly pathogenic Y. enterocolitica, low pathogenic Y. enterocolitica, and Y. pseudotuberculosis.
Specific primers and probes were created from three distinct genes to differentiate these Yersinia groups and were tested on 26 strains to ensure specificity.
The developed methods proved effective in detecting low levels of Yersinia DNA in various samples, enhancing capabilities for monitoring and understanding the epidemiology of these pathogens.
From July 2017 to Jan 2019, a total of 572 retail fresh vegetables were collected to clarify the contamination of Salmonella in the Mekong Delta, Vietnam. Salmonella was isolated from 74 (12.9%) of 572 samples.