Publications by authors named "Hidenori Hiranuma"
Article Synopsis
- The study explores the importance of cell alignment in human heart muscle for effective tissue movement, focusing on hiPSC-derived cardiomyocytes (CMs) grown on microstripe-patterned plates.
- Aligned cardiomyocytes (ACMs) showcased adult-like characteristics in terms of pathology, gene expression, and drug response compared to unaligned CMs.
- The research suggests that ACMs could serve as a better model for drug testing and offer potential for regenerative therapies in heart failure, particularly using cells from patients with hypertrophic cardiomyopathy (HCM).
Mitochondria combine the production of energy with an efficient chain of reduction-oxidation (redox) reactions but also with the unavoidable production of reactive oxygen species. Oxidative stress leading to mitochondrial dysfunction is a critical factor in many diseases, such as cancer and neurodegenerative and lifestyle-related diseases. Effective antioxidants thus offer great therapeutic and preventive promise.
View Article and Find Full Text PDFBackground: Human interferon-gamma (hIFN-gamma) is produced by lymphocytes and has a variety of biological properties. Measurement of hIFN-gamma is widely used for various immunological responses for allergic or autoimmune diseases. Enzyme-linked immunosorbent assay (ELISA) is an established immunoassay used to quantify cellular metabolites or cytokines.
View Article and Find Full Text PDFAn automated fluorescence protein sequencer using 7-methylthio-5-(2,1,3-benzoxadiazolyl) isithiocyanate (MTBD-NCS), a fluorescent Edman reagent, is developed by the modification of a commercial protein sequencer. The generated MTBD-thiohydantoin amino acids fluoresced strongly, whereas the by-products such as MTBD-thiocarbamoyl amino acids and MTBD-carbamoly amino acids did not fluoresce. A few interfering peaks were observed in the chromatogram and amino acid sequence was easily determined.
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