Publications by authors named "Hideki Tokushige"
Article Synopsis
- - This study examined the effects of azithromycin (AZM) in treating ocular surface infections by focusing on its anti-inflammatory and anti-virulence properties against Staphylococcus aureus-induced keratitis in rabbits.
- - The results showed that higher doses of AZM significantly reduced both clinical scores of keratitis and the viable bacterial counts in the cornea, indicating effective treatment for the infection.
- - Additionally, AZM demonstrated the ability to inhibit inflammation and bacterial toxin production, suggesting that its therapeutic benefits extend beyond just killing bacteria.
Glaucoma is a chronic optic neuropathy that leads to visual field loss. Elucidating the mechanisms underlying glaucoma is essential for developing new treatments, such as neuroprotective drugs. Various glaucoma models based on the induction of intraocular pressure (IOP) elevation have been established for use in glaucoma studies.
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- This study investigates how the pH of brimonidine ophthalmic solutions affects its distribution in the posterior ocular tissues of pigmented rabbits after topical administration.
- The research involved analyzing tissue samples at various time points post-administration of two different pH solutions (6.4 and 7.3) to compare brimonidine concentrations.
- Results show that while both pH levels lead to significant drug bioavailability, the increase from pH 6.4 to 7.3 boosts brimonidine levels in posterior ocular tissues, suggesting similar absorption in the aqueous humor.
Article Synopsis
- The study investigates how the concentration of brimonidine, an eye drop medication, in the vitreous (the gel-like substance in the eye) relates to its free concentration in the retina/choroid (the target area for the drug).
- Researchers conducted experiments on pigmented rabbits to measure brimonidine levels after administering the drops and studied its binding to melanin in the eye tissues.
- Results showed that the concentration of brimonidine in the vitreous is closely linked to the free concentration in the retina/choroid, suggesting that vitreous concentration can serve as an indicator of drug levels in this target area.
Purpose: To evaluate the pharmacokinetics and efficacy of topically applied nonsteroidal anti-inflammatory drugs (NSAIDs) in the retinochoroidal tissues of rabbits.
Methods: The cyclooxygenase (COX) inhibitory activity of diclofenac, bromfenac, and amfenac, an active metabolite of nepafenac, were determined using human-derived COX-1 and COX-2. Each of the three NSAIDs was applied topically to rabbits, and after 0.
The aim of this study was to verify the correlation between mean blur rate (MBR) obtained with CCD-equipped laser speckle flowgraphy (LSFG) and capillary blood flow (CBF) obtained by the hydrogen gas clearance method in rabbit optic nerve head (ONH). Using Japanese white rabbits under systemic anesthesia, a hydrogen electrode was inserted an area of the ONH free from superficial capillaries. MBR was measured with LSFG near the hydrogen electrode.
View Article and Find Full Text PDFPurpose: To investigate the effects of Y-39983, a selective Rho-associated coiled coil-forming protein kinase inhibitor, on blood flow in the optic nerve head (ONH) in rabbits and axonal regeneration of retinal ganglion cells (RGCs) in rats.
Methods: Blood flow in ONH was measured by the laser speckle method after topical administration of 0.05% Y-39983 solution or its vehicle in rabbit eyes.
Objective: To investigate the effects and safety of topical administration of an ophthalmic solution of a selective Rho-associated coiled coil-forming protein kinase (ROCK) inhibitor, SNJ-1656, 0.003% to 0.1%, in healthy male adult volunteers.
View Article and Find Full Text PDFAim: To determine the anti-inflammatory activity of gatifloxacin in ophthalmic use.
Methods: The following 3 experiments were carried out. (1) Rabbits were inoculated intracorneally with methicillin-resistant Staphylococcus aureus and topically treated with gatifloxacin or levofloxacin.
Purpose: To elucidate the intraocular pressure (IOP)-lowering effects and associated characteristics of Y-39983, a selective Rho-associated coiled coil-forming protein kinase (ROCK) inhibitor derived from Y-27632, in animal eyes.
Methods: Y-39983 was compared with Y-27632 for selectivity of ROCK inhibition by biochemical assay. The IOP was monitored by pneumatonometer in albino rabbits and cynomolgus monkeys that were given topically administered Y-39983.
Purpose: To evaluate the association between tear mucin and dry eye, we compared tear fluid sialic acid concentration in dry eye patients with that in normal volunteers. We also compared the sialic acid concentration with the results of several different types of dry eye examinations.
Methods: Subjects comprised 16 age-matched, normal healthy controls and 45 dry eye patients.
This study was designed to determine the most effective dose of gatifloxacin in ophthalmic solution for control of methicillin-resistant Staphylococcus aureus (MRSA) corneal infections in rabbits. Rabbits were inoculated by injecting 9300 colony-forming units of MRSA into the corneal stroma of the eye (n=43). They were then randomly assigned to topical administration of saline, ofloxacin 0.
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