Publications by authors named "Hf Ouyang"
Background: Homologous recombination deficiency (HRD) is a well-known biomarker which could predict poly-ADP ribose polymerase 1 (PARP) inhibitor and platinum drug response. As an aggressive cancer, small-cell lung cancer (SCLC) is sensitive to platinum drugs, but relapse occurs rapidly. Herein, we aim to illustrate the genomic alteration patterns of homologous recombination repair (HRR)-related genes in a Chinese SCLC cohort and further analyze the relationship among HRR gene mutations and known biomarkers of immune checkpoint inhibitor (ICI) response, including tumor mutation burden (TMB) and programmed cell death-ligand 1 (PD-L1) expression.
View Article and Find Full Text PDFThis study aims to establish a rapid and sensitive UPLC-MS/MS method for simultaneously determining the content of strychnine and paeoniflorin in plasma and brain tissue of rats, and compare the pharmacokinetic behavior and brain tissue distribution of paeoniflorin combined with normal and toxic doses of strychnine in rats after percutaneous administration. Compared with those in the toxic-dose strychnine group, the AUC_(0-t), AUC_(0-∞), and C_(max) of strychnine decreased by 51.51%, 45.
View Article and Find Full Text PDFOperation of RF-driven negative hydrogen ion source in China Spallation Neutron Source.
Rev Sci Instrum
November 2019
The China Spallation Neutron Source started delivering neutron beams to users in March 2018. To upgrade the beam power to 500 kW and improve the performance of the ion source, an RF-driven negative hydrogen (H) ion source is under development. The source has a silicon nitride ceramic plasma chamber surrounded by a 4.
View Article and Find Full Text PDFArticle Synopsis
- Researchers explored the use of Erythropoietin (EPO) gene-modified mesenchymal stem cells (MSCs) to combat airway remodeling in asthma, finding that these modified cells exhibited greater therapeutic effects compared to unmodified MSCs.
- The study involved creating EPO-MSCs via a lentivirus vector, identifying their characteristics, and testing their impact on asthmatic mice through various analyses including histological evaluations and cytokine measurements.
- Results indicated that EPO-MSCs significantly reduced airway inflammation, cell proliferation, and remodeling factors, potentially by inhibiting the TGF-β1-TAK1-p38MAPK signaling pathway.
Article Synopsis
- Chronic bronchial asthma leads to persistent airway inflammation and structural changes, making it hard to treat with existing drugs.
- Interleukin-13 (IL-13) and IL-25 are key players in this inflammation and remodeling process.
- Research showed that targeting both IL-13 and IL-25 at the same time reduced inflammation and tissue changes more effectively than treating with either one alone, suggesting a new potential treatment approach for asthma patients who don't respond well to current therapies.
Article Synopsis
- The study investigates KyoT2's role in asthma, focusing on airway remodeling and hyperresponsiveness.
- KyoT2, which inhibits Notch signaling, was tested on asthmatic mice to assess its effects on airway structure and resistance.
- Results showed that KyoT2 reduces Hes1 expression, lessens airway remodeling, and improves hyperresponsiveness, suggesting it could be a viable treatment for asthma.
Notch signaling regulates col1α1 and col1α2 expression in airway fibroblasts.
Exp Biol Med (Maywood)
December 2014
Subepithelial fibrosis is one of the common pathological features of asthmatic airway remodeling. During subepithelial fibrosis, type I collagen becomes the most abundant extracellular protein component. Studies have shown that Notch signaling participates in the progression of fibrosis; however, whether Notch signaling is involved in regulating type I collagen expression in airway fibroblasts remains unclear.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the effects of recombinant bacille Calmette-Guerin (rBCG) that expresses Der p2 on asthma, particularly its influence on Th17 cell differentiation and airway inflammation.
- Results showed that transferring dendritic cells infected with Der p2 rBCG led to reduced airway inflammation and mucin production, effectively inhibiting excessive Th17 responses compared to traditional BCG.
- The research highlights the importance of dendritic cells in rBCG's immunoregulatory functions and provides insight into the cellular mechanisms behind its potential as an asthma treatment.
Article Synopsis
- Notch signaling is shown to regulate the expression of MUC5AC, a key mucin in asthma-related mucus overproduction.
- Various experiments revealed that activation of Notch can enhance MUC5AC promoter activity while Hes proteins can repress it.
- This study suggests that targeting the Notch signaling pathway could provide new therapeutic options for managing mucus overproduction in asthma.
Article Synopsis
- Researchers studied a recombinant bacille Calmette-Guérin (rBCG) vaccine expressing the Der p 2 allergen, which showed promise in reducing asthma symptoms by manipulating immune responses.
- The study aimed to investigate the role of dendritic cells (DCs) in this process and involved transferring DCs from vaccinated mice to other mice to assess their impact on airway inflammation.
- Results indicated that the transferred DCs significantly reduced asthmatic responses and promoted the development of regulatory T cells, suggesting that DCs are crucial for the rBCG's anti-asthma effects, especially through enhanced recruitment of certain DC subtypes.
Suppression of allergic airway inflammation in a mouse model of asthma by exogenous mesenchymal stem cells.
Exp Biol Med (Maywood)
December 2011
Article Synopsis
- Mesenchymal stem cells (MSCs) show potential in reducing acute lung inflammation and fibrosis, but their effectiveness in allergic asthma is less understood.
- MSCs were introduced into a mouse model of asthma, and their migration to inflamed lung tissues was tracked, revealing their movement depends on a specific chemical signal.
- The study found that MSCs help mitigate allergic inflammation by promoting a shift from a T-helper 2 (Th2) to a T-helper 1 (Th1) immune response, suggesting MSC-based therapies could be beneficial for asthma treatment.
Article Synopsis
- The study investigates the role of bone marrow-derived adult stem cells in the airway remodeling process seen in asthma, which involves chronic inflammation and abnormal cell growth.
- Using mice models, researchers established a chronic asthma condition and observed the presence of fluorescently labeled bone marrow cells in lung tissues.
- The results indicate that a significant number of these cells contributed to the production of collagen and muscle in the lungs, suggesting they play a key role in asthma-related airway changes.
Article Synopsis
- Thousands have died from HCV due to lack of vaccines and effective therapies, highlighting the need for new treatments.
- Cytotoxic T lymphocytes are key in the immune response against HCV, and combining T helper epitopes from tetanus toxin with HCV core protein may enhance vaccine development efforts.
- Research showed that modified plasmids increased antibody production and immune response in mice, suggesting a promising strategy for creating HCV vaccines.
The goal of this study was to evaluate the effect of the Na+/H+ exchanger-1 (NHE-1) antisense gene on drug-resistant human small cell lung cancer (SCLC) cell proliferation and apoptosis. A recombinant NHE-1 antisense gene was transfected into drug-resistant human SCLC H446/CDDP cells. Intracellular pH (pHi) was measured with fluorescence spectrophotometry.
View Article and Find Full Text PDFArticle Synopsis
- Allergic asthma is driven by T helper (Th)2 cell immune responses, and new immunotherapy strategies aim to shift these responses towards Th1 to alleviate symptoms.
- Previous studies demonstrated that a genetically modified form of bacille Calmette-Guerin (rBCG) could induce such a shift in naive mice, but its effectiveness in reducing allergic airway inflammation needed further investigation.
- The current research showed that rBCG significantly reduced airway inflammation and related symptoms in allergic mice by promoting a Th1 response and enhancing regulatory T cells that suppress Th2 cells, indicating its potential as an effective asthma treatment.
Prospero-related homeobox protein (Prox1) plays essential roles in the development of many tissues and organs. In the present study, we show that Prox1 is modified by the small ubiquitin-like protein SUMO-1 in cultured cells. Mutation analysis identified at least four potential sumoylation sites within the repression domain of Prox1.
View Article and Find Full Text PDFEvidence has shown that Notch signaling modulates CD4(+)CD25(+) regulatory T-cells (Tregs). As transcription factor Foxp3 acts as a master molecule governing the development and function of Tregs, we investigated whether Notch signaling might directly regulate Foxp3 expression. Here, we provide evidence that Notch signaling can modulate the FOXP3 promoter through RBP-J- and Hes1-dependent mechanisms.
View Article and Find Full Text PDF[Expression of apoptosis related protein in transforming growth factors-beta signaling pathway and its effects on the cell apoptosis in the lung tissues after acute pulmonary embolism].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
June 2008
Article Synopsis
- The study aims to investigate changes in the expression of the apoptosis-related protein ARTS in lung tissues following acute pulmonary embolism (APE) in rats, focusing on how it affects cell apoptosis.
- Methods included creating a rat model of APE, collecting lung tissue samples at different times, measuring ARTS and related protein levels using techniques like RT-PCR and Western blotting, and identifying apoptotic cells through the TUNEL method.
- Results showed significant increases in ARTS mRNA and protein levels at specific time points after APE, with a notable presence in bronchial and alveolar epithelial cells, alongside a rise in apoptosis levels and a decrease in anti-apoptotic proteins compared to healthy controls.
Brownian motion in a singular potential and a fractal renewal process.
Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics
October 1995
Scaling structure and 1/f noise in one-dimensional surface-reaction models.
Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics
January 1995
1/f noise and one-dimensional Brownian motion in a singular potential.
Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics
October 1994
Two-dimensional sandpile model with stochastic slide.
Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics
October 1993