Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA.

A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. The PEIA process was primer based on a template to continuously extend multiple repeat sequences, using DNA polymerase, to form long ssDNAs. M13mp18 ssDNA was then coupled to multiple long ssDNAs from PEIA and functionalized in a signal tag DNA nanostructure (STDN) for the lateral flow biosensor.