An electrochemical biosensor for sensitive detection of live Salmonella in food via MXene amplified methylene blue signals and electrostatic immobilization of bacteriophages.

A novel bacteriophage-targeted electrochemical biosensor designed for accurate and quantitative detection of live Salmonella in food samples is presented. The biosensor is simply constructed by electrostatic immobilizing bacteriophages on MXene-nanostructured electrodes. MXene, renowned for its high surface area, biocompatibility, and conductivity, serves as an ideal platform for bacteriophage immobilization.

Photoelectrochemical/Colorimetric Dual-Mode Specific Detection of Based on the Enzymatic Reaction Triggered by Catalase from Lysed Bacteria.

() is abundant in nature and frequently leads to various health issues. Bacteriophages as obligate intracellular parasites of bacteria have the ability to specifically identify and infect , causing bacterial lysis and the release of endogenous catalase (CAT). The released CAT triggers the conversion of HO into O and HO, resulting in a notable decrease in UV absorption at 570 nm and a concurrent surge in photocurrent.

Diverse Genotypes of spp. Associated with Dairy Farm Systems in Jiangsu and Shandong Provinces in China.

spp. are the most concerning foodborne pathogen in infant formula milk powder. Currently, there are many reports on the prevalence of spp.

Genomic epidemiology and characterization of isolates from raw milk in Jiangsu, China: emerging broader host tropism strain clones ST59 and ST398.

is highly pathogenic and can cause disease in both humans and domestic animals. The aim of this study was to investigate the genomic epidemiology of isolates from raw milk in Jiangsu Province, China, to identify predominant lineages and their associated genomic and phenotypic characteristics. In this study, we identified 117 isolates collected from 1,062 samples in Jiangsu Province between 2021 and 2022.

MXene/zinc ion embedded agar/sodium alginate hydrogel for rapid and efficient sterilization with photothermal and chemical synergetic therapy.

Bacterial infections can significantly impair wound healing. Therefore, it is essential to develop wound dressings with high antimicrobial activity. Hydrogels are often used as wound dressings due to their excellent physicochemical properties.

Solid-state fermentation with RT-3 enhanced the nutritional properties of soybeans.

Fermented soybean products are favorite foods worldwide because of their nutritional value and health effects. In this study, solid-state fermentation (SSF) of soybeans with RT-3 was performed to investigate its nutraceutical potential. A rich enzyme system was released during SSF.

Antiviral effect of a bacteriophage on murine norovirus replication via modulation of the innate immune response.

Bacteriophages (phages) are viruses of bacteria. Despite the growing progress in research on phage interactions with eukaryotic cells, our understanding of the roles of phages and their potential implications remains incomplete. The objective of this study was to investigate the effects of the Staphylococcus aureus phage vB_SauM_JS25 on murine norovirus (MNV) replication.

Biodiversity of New Lytic Bacteriophages Infecting spp. in Freshwater Environment.

Bacteriophages, viruses that infect and replicate within prokaryotic cells are the most abundant life forms in the environment, yet the vast majority of them have not been properly reported or even discovered. Almost all reported bacteriophages infecting the family, with being the major subject of studies, have been isolated from wastewater, sewage, and effluent resources. In the present study, we focused on the distribution and biodiversity of phages in an aquatic ecosystem.

Stabilization of Liposomes by Perfluorinated Compounds.

Perfluorinated compounds (PFCs) are emerging persistent environmental contaminants that may be toxic to animals and humans. To gain fundamental insights into the mechanism of their toxicity, the interactions of phosphocholine (PC) liposomes as model membranes were studied with three types of PFCs, including perfluorooctanoic acid, perfluorooctane sulfonate, and perfluorohexanesulfonic acid potassium salt, together with three common surfactants: sodium dodecyl sulfate (SDS), cetyltrimethylammonium bromide (CTAB), and sodium 1-heptanesulfonate (SHS). The interactions were systematically characterized by zeta potential measurement, dynamic light scattering, negative-stain transmission electron microscopy, and fluorescence spectroscopy.

Paper-based immune-affinity arrays for detection of multiple mycotoxins in cereals.

Mycotoxins produced by different species of fungi may coexist in cereals and feedstuffs, and could be highly toxic for humans and animals. For quantification of multiple mycotoxins in cereals, we developed a paper-based mycotoxin immune-affinity array. First, paper-based microzone arrays were fabricated by photolithography.

Development of immune-affinity 96 spots monolith array for multiple mycotoxins detection in food samples.

In this paper, a novel highly sensitive chemiluminescence immune-affinity 96 spots monolith array was developed to detect deoxynivalenol (DON), zearalenone (ZEN), T-2 toxin (T-2), and fumonisin B1 (FB1) in corn samples. Firstly, the monolith array was prepared through on suit UV-initiated copolymerization using polyethylene glycol diacrylate (PEGDA) as cross-linker, glycidyl methacrylate (GMA) as functional monomer and polyethylene glycol 200 (PEG 200) as the porogen. Subsequently, the four mycotoxins immune-affinity monolith array was prepared by immobilization of DON, ZEN, T-2, and FB1 antibody.

Separation and analysis of cis-diol-containing compounds by boronate affinity-assisted micellar electrokinetic chromatography.

Cis-diol-containing compounds (CDCCs) are usually highly hydrophilic compounds and are therefore difficult to separate by conventional reversed-phase-based micellar electrokinetic chromatography (MEKC) due to poor selectivity. Here, we report a new method, called boronate affinity-assisted micellar electrokinetic chromatography (BAA-MEKC), to solve this issue. A boronic acid with a hydrophobic alkyl chain was added to the background electrolyte, which acted as a modifier to adjust the selectivity.

Probing the interactions between boronic acids and cis-diol-containing biomolecules by affinity capillary electrophoresis.

The affinity of boronic acids to cis-diol-containing biomolecules has found wide applications in many fields, such as sensing, separation, drug delivery, and functional materials. A sound understanding of the binding interactions will greatly facilitate exquisite applications of this chemistry. Although a few analytical tools have been available for the characterization of the interactions, these techniques are associated with some apparent drawbacks, so they are only applicable to a limited range of boronic acids and cis-diol-containing biomolecules.

Preparation of organic-silica hybrid boronate affinity monolithic column for the specific capture and separation of cis-diol containing compounds.

A new boronate-silica hybrid monolithic column was prepared using a one-pot approach with 3-acrylamidophenylboronic acid (AAPBA) as the boronate affinity ligand. The AAPBA-silica monolith exhibited several attractive advantages. First, it is highly hydrophilic, providing excellent specificity and avoiding the presence of organic solvent in the mobile phase.

A benzoboroxole-functionalized monolithic column for the selective enrichment and separation of cis-diol containing biomolecules.

A benzoboroxole-functionalized monolithic column was synthesized, which exhibited the best specificity and affinity towards cis-diol containing biomolecules as compared with the boronate affinity monolithic columns reported as well as significant secondary separation capability under acidic conditions.

Immuno-magnetic beads-based extraction-capillary zone electrophoresis-deep UV laser-induced fluorescence analysis of erythropoietin.

Erythropoietin (EPO) is an important glycoprotein hormone. Recombinant human EPO (rhEPO) is an important therapeutic drug and can be also used as doping reagent in sports. The analysis of EPO glycoforms in pharmaceutical and sports areas greatly challenges analytical scientists from several aspects, among which sensitive detection and effective and facile sample preparation are two essential issues.