Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects.

Objective: The aim of this study was to investigate the effect of sperm DNA fragmentation on fertilization rate, embryo development (blastulation rate), and pregnancy outcomes for ICSI cycles performed in a cohort of couples using donor eggs and to assess the remaining embryos that were not transferred or frozen for apoptotic markers.

Methods: Eighty-two women (egg recipients) were included in the study (2016) were included in the study. The recipients' mean age was 41.

Changes in DNA fragmentation during sperm preparation for intracytoplasmic sperm injection over time.

Objective: To compare the DNA fragmentation of semen samples established by terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) after incubation in polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) for different time periods.

Design: Comparative prospective study.

Setting: Center for reproductive medicine.