Hydrogen Sulfide-Triggered Artificial DNAzyme Switches for Precise Manipulation of Cellular Functions.

The development of synthetic molecular tools responsive to biological cues is crucial for advancing targeted cellular regulation. A significant challenge is the regulation of cellular processes in response to gaseous signaling molecules such as hydrogen sulfide (HS). To address this, we present the design of Gas signaling molecule-Responsive Artificial DNAzyme-based Switches (GRAS) to manipulate cellular functions via HS-sensitive synthetic DNAzymes.

Visualization of Protein-Specific Glycation in Living Cells via Bioorthogonal Chemical Reporter.

Due to the lack of suitable chemical tools, probing the protein-specific glycation is highly challenging. Herein, we present a strategy based on glycation chemical reporter and proximity-induced FRET signal readout for visualizing protein-specific glycation in living cells. We first developed a bioorthogonal glucose analogue, 6-azido-6-deoxy-D-glucose (6AzGlc), as a novel glycation chemical reporter.

Oligonucleotide Discrimination Enabled by Tannic Acid-Coordinated Film-Coated Solid-State Nanopores.

Discrimination of nucleotides serves as the basis for DNA sequencing using solid-state nanopores. However, the translocation of DNA is usually too fast to be detected, not to mention nucleotide discrimination. Here, we utilized polyphenolic TA and Fe, an attractive metal-organic thin film, and achieved a fast and robust surface coating for silicon nitride nanopores.

One-Step In Situ Self-Assembly of Biodegradable Films for Long-Term Intravesical Bladder Cancer Therapy.

Intravesical instillation therapy is increasingly recognized as one of the most common clinical treatment strategies for bladder cancer. However, the antitumor efficacy of chemotherapy drugs is still limited due to their rapid clearance by periodic urination. To circumvent this issue, a drug-loaded thin film comprising the self-assembly of tannic acid (TA) and ferric ions (Fe) was in situ fabricated on the bladder wall in vivo.

DNA-Templated Glycan Labeling for Monitoring Receptor Spatial Distribution in Living Cells.

Tracking the spatial distribution of receptor tyrosine kinases in their native environment contributes to understanding the homeostatic or pathological states at a molecular level. Conjugation of DNA tags to a specific receptor is a powerful tool for monitoring receptor spatial distribution. However, long-term stable trafficking in live cells without interfering with the intrinsic receptor function remains a challenge.

Detection and Imaging of Hydrogen Sulfide in Lysosomes of Living Cells with Activatable Fluorescent Quantum Dots.

The simple, sensitive, and specific detection of hydrogen sulfide (HS) is of great importance because of its crucial role in food safety, environmental pollution, and various pathological and physiological processes. Here, we reported activatable fluorescence nanoprobe-based quantum dots (QDs) for sensitive and selective monitoring of HS in red wine, environmental water samples, and lysosome of live cancer cells. The nanoprobe was prepared through a strong electrostatic interaction between thioglycolic-acid-stabilized CdTe QDs and p-amino thiophenol capped silver nanoparticles (AgNPs) that resulted in the formation of the assembled nanostructure, called QD/AgNP nanocomplexes.