Phytochemical and biological assessment of secondary metabolites isolated from a rhizosphere strain, Sphingomonas sanguinis DM of Datura metel.

Background: The plant roots excrete a large number of organic compounds into the soil. The rhizosphere, a thin soil zone around the roots, is a hotspot for microbial activity, making it a crucial component of the soil ecosystem. Secondary metabolites produced by rhizospheric Sphingomonas sanguinis DM have sparked significant curiosity in investigating their possible biological impacts.

Revealing the diversity of Jojoba-associated fungi using amplicon metagenome approach and assessing the in vitro biocontrol activity of its cultivable community.

Jojoba shrubs are wild plants cultivated in arid and semiarid lands and characterized by tolerance to drought, salinity, and high temperatures. Fungi associated with such plants may be attributed to the tolerance of host plants against biotic stress in addition to the promotion of plant growth. Previous studies showed the importance of jojoba as jojoba oil in the agricultural field; however, no prior study discussed the role of jojoba-associated fungi (JAF) in reflecting plant health and the possibility of using JAF in biocontrol.

Neuroprotective activity of (L.) Schott leaves against monosodium glutamate-induced excitotoxicity in rats: phytochemical and molecular docking study.

(L.) Schott is a food crop with long history of use in treatment of various disorders including neurological diseases. The methanolic leaves extract (ME) and its -butanol fraction (-BF) demonstrated significant neuroprotective activity in monosodium glutamate induced excitotoxicity in rats.

Antimicrobial, antiproliferative activities and molecular docking of metabolites from Alternaria alternata.

Endophytic fungi allied to plants have sparked substantial promise in discovering new bioactive compounds. In this study, propagation of the endophytic fungus Alternaria alternata HE11 obtained from Colocasia esculanta leaves led to the isolation of Ergosterol (1), β-Sitosterol (2), Ergosterol peroxide (3), in addition to three dimeric naphtho-γ-pyrones, namely Fonsecinone A (4), Asperpyrone C (5), and Asperpyrone B (6), which were isolated from genus Alternaria for the first time. Structures of the isolated compounds were established on the basis of extensive 1D and 2D NMR and, MS measurements.

Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds.

Background: Cadmium is an environmentally toxic metal that has deleterious effects on both animals and humans due to its accumulation in different body tissues. Physalis peruviana L. fruit and calyx contain many active constituents which are used traditionally for their different biological activities.

RF-3192C and other polyketides from the marine endophytic ASSB4: structure assignment and bioactivity investigation.

Chemical investigation of the methanolic extract of endophytic SB4, isolated from the marine alga , afforded the pentacyclic polyketide, RF-3192C (), the dimeric coumarin orlandin (), fonsecin B (), TMC-256A1 (), cyclo-(Leu-Ala) (), and cerebroside A ().The chemical structure of RF-3192C () is assigned herein for the first time using 1D/2D NMR and HRESI-MS. Additionally, the revision of the NMR assignments of orlandin () was reported herein as well.

Meleagrin from marine fungus Nq45: crystal structure and diverse biological activity studies.

The crystal structure and unambiguous absolute configuration of meleagrin () isolated from fungus Nq45 is reported herein to first time on the bases of single crystal X-ray diffraction. Together with , haenamindole (), isorugulosuvine (), secalonic acid D (), ergosterol () and cerebroside A () were obtained and their structures were determined by ESI MS and NMR data analysis. Diverse biological activity of meleagrin () was investigated.

Terretonin O: a new meroterpenoid from .

Terretonin O (), a new meroterpenoid, was isolated individually from both methanolic extracts of thermophilic TM8 and marine LGO13. The recently reported terretonins M () and N ( were further isolated from the fungus LGO13 along with nine known compounds, terrelumamide A (), terrein (), methyl-3,4,5-trimethoxyl-2-[2-(nicotinamide)benzamido] benzoate (), butyrolactones I-III (-), aspulvinone O (), ergosterol, ergost-4-ene-3-one and methyl linoleate. Structure of terretonin O () was established on the bases of HRESIMS, 1D and 2D NMR spectra and comparison with its analogues in literatures.

Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation.

Background: Cucumis prophetarum var. prophetarum is used in Saudi folk medicine for treating liver disorders and grows widely between Abha and Khamis Mushait City, Saudi Arabia.

Methods: Bioassay-guided fractionation and purification were used to isolate the main active constituents of Cucumis prophetarum var.

Iridoid and lignan glycosides from Citharexylum spinosum L.

From the aerial parts of Citharexylum spinosum L., one new iridoid glucoside, the 7-ss-O-acetate (1) of lamiide, along with four known iridoid glucosides, lamiide (2), lamiidoside (3), duranterectoside C (4), 8-epiloganin (5) and one known lignan glucoside (+)-lyonirenisol-3a-O-ss-D-glucopyranoside (6) were isolated. The compound structures were established by one- and two-dimensional 1H- and 13C-NMR as well as by ESI-MS spectra.

Cembranoid diterpenes and a briarane diterpene from corals.

Two new cembrane diterpenes (+)-polydactylide (1) and (+)-7alpha,8beta-dihydroxydeepoxysarcophine (2) together with (+)-sarcophine (3) and (+)-sarcophytoxide (4) have been isolated from the soft coral Sinularia polydactyla. The new cembrane diterpene (-)-7beta-hydroxy-8alpha-methoxydeepoxysarcophine (5) has been obtained from the soft coral Sarcophyton trocheliophorum and the known briarane diterpene briaexcavatolide W (6) from the gorgonian coral Briareum sp.The structures were determined primarily by NMR spectroscopy.

Saponins from the seeds of Mimusops laurifolia.

Nine saponins were isolated from the seeds of Mimusops laurifolia. Their structures were established using one- and two-dimensional NMR spectroscopy and mass spectrometry. Three of them are identified as: 3-O-(beta-d-apiofuranosyl-(1-->3)-beta-d-glucuronopyranosyl)-28-O-(alpha-l-rhamnopyranosyl-(1-->3)-beta-d-xylopyranosyl-(1-->4)-alpha-l-rhamnopyranosyl-(1-->2)-alpha-l-arabinopyranosyl)-16alpha-hydroxyprotobassic acid, 3-O-(beta-d-glucopyranosyl-(1-->3)-beta-d-glucopyranosyl)-28-O-(alpha-l-rhamnopyranosyl-(1-->3)-beta-d-xylopyranosyl-(1-->4)-alpha-l-rhamnopyranosyl-(1-->2)-alpha-l-arabinopyranosyl)-16alpha-hydroxyprotobassic acid and 3-O-(beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl)-28-O-(alpha-l-rhamnopyranosyl-(1-->3)-beta-d-xylopyranosyl-(1-->4)-alpha-l-rhamnopyranosyl-(1-->2)-alpha-l-arabinopyranosyl)-16alpha-hydroxyprotobassic acid.

Glycosides from Bougainvillea glabra.

Three glycosides were isolated from Bougainvillea glabra and their structures were determined by extensive use of 1D and 2D NMR spectroscopy ((1)H and (13)C). First compound was identical to momordin IIc (quinoside D) [beta-D-glucopyranosyl 3-O-[beta-D-xylopyranosyl-(1 --> 3)-O-(beta-D-glucopyranosyluronic acid)] oleanolate], second compound was quercetin 3-O-alpha-L-(rhamnopyranosyl)(1 --> 6)-[alpha-L-rhamnopy-ranosyl(1 --> 2)]-beta-D-galactopyranoside and third compound was its derivative quercetin 3-O-alpha-L-(4-caffeoylrhamnopyranosyl)(1 --> 6)-[alpha-L-rhamnopyranosyl (1 --> 2)]-beta-D-galactopyranoside, a new natural product.

Saponins from the leaves of Mimusops laurifolia.

Fifteen new bidesmosidic triterpenoid saponins (1-15) were isolated from a methanol extract of the leaves of Mimusops laurifolia. Their structures were established using one- and two-dimensional NMR spectroscopy and mass spectrometry and determined to be bidesmosides of protobassic acid (2-4, 11, 12, and 15) and of 16alpha-hydroxyprotobassic acid (1, 5-10, 13, and 14).

Sablacaurin A and B, two 19-nor-3,4-seco-lanostane-type triterpenoids from Sabal causiarum and Sabal blackburniana, respectively.

In search for bioactive compounds from Sabal species, sablacaurin A [25-ethyl,23-methyl-19-nor-24-methylene-3,4-seco-4(28)-lanosten-10,3-olide] and sablacaurin B [24-ethyl,24-methyl-19-nor-3,4-seco-4(28),25(26)-lanostadiene-10,3-olide], the first 19-nor lanostane derivatives of the 3,4-seco type with a spiro element, have been isolated from the leaves of Sabal causiarum and Sabal blackburniana respectively, together with the known squalene (S. blackburniana) and ss-sitosterol (S. causiarum).