Publications by authors named "Hernandez-Castro R"

Nocardia spp are ubiquitous, gram-positive, variably acid-fast, branching and beaded filamentous, facultative intracellular bacteria that are resistant to phagocytosis and can cause localized or systemic disease in a variety of mammals, including humans, as well as in birds, fish and reptiles. Seventeen pet domestic ferrets (Mustela putorius furo) were diagnosed with nocardiosis by several methods including cytological evaluation, histopathology, Ziehl-Neelsen staining and polymerase chain reaction (PCR). All except two ferrets were 2 years old or older at the time of clinical presentation.

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  • - Rickettsia spp. are harmful bacteria that pose risks to public health and livestock production, leading to losses in meat and dairy quality, making their prevention and early treatment crucial.
  • - The study involved collecting hard ticks from Bos indicus cattle in Mexico, identifying them through morphological methods and genetic sequencing.
  • - Results showed the presence of Rickettsia amblyommatis in Amblyomma mixtum and Rhipicephalus microplus ticks, indicating the pathogen circulates in local livestock and calling for further research.
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Tropicoporus tropicalis (formerly Phellinus tropicalis) is a saprophytic basidiomycete that has been implicated in refractory mycoses in humans, particularly in patients with chronic granulomatous disease. Despite its clinical significance, T. tropicalis is an under-recognised cause of eumycetoma, with no prior reports available.

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The present report describes a case of acanthocephaliasis associated with necrotic enteritis in quetzals (Pharomachrus mocinno). Three juvenile quetzals exhibited depression, anorexia, ruffled feathers, and sudden death. They were submitted to the laboratory for examination.

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  • - Mycetoma is a chronic inflammatory disease affecting subcutaneous tissues and sometimes bones, often caused by bacteria from the genus Nocardia.
  • - A 43-year-old male presented with extensive mycetoma lesions on his forearm, chest, and neck, showing signs such as erythema and draining pus, diagnosed through genetic sequencing which identified Nocardia mexicana.
  • - Successful treatment with a combination of antibiotics was achieved after six months, and the case suggests that Nocardia mexicana can spread through contact with animals, as it was linked to the patient’s manipulation of a calf.
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, an obligate intracellular bacterium, is the etiological agent of Q fever in humans and one of the causes of abortion in small ruminants. Although coxiellosis is considered an exotic disease, there are a few reports in Mexico. The objective of this work was to determine the presence of .

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  • Hospital-acquired severe infections are linked to genetic factors related to antibiotic resistance, specifically in strains that may carry genes for carbapenem and tigecycline resistance.
  • A study analyzed 384 genomes from clinical and non-clinical sources globally, revealing significant variability in genetic content, with antibiotic resistance genes largely found in the accessory genome.
  • The research indicates that while core genomes of environmental and hospital isolates are similar, the hospital strains acquire antibiotic resistance genes due to high selective pressure, with no geographical bias in their distribution.
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  • The study explores the characteristics of uropathogenic E. coli (UPEC) associated with recurrent urinary tract infections (RUTIs), focusing on their antimicrobial resistance.
  • Researchers analyzed 140 strains from 70 outpatients suffering from RUTIs to determine whether infections were due to relapse or reinfection.
  • Findings revealed that most cases (64.2%) were relapses linked to the original strain, which exhibited significantly higher levels of antimicrobial resistance compared to reinfecting strains, with 89% being multidrug-resistant.
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The dot-blot is a simple, fast, sensitive, and versatile technique that enables the identification of minimal quantities of DNA specifically targeted by probe hybridization in the presence of carrier DNA. It is based on the transfer of a known amount of DNA onto an inert solid support, such as a nylon membrane, utilizing the dot-blot apparatus and without electrophoretic separation. Nylon membranes have the advantage of high nucleic acid binding capacity (400 µg/cm), high strength, and are positively or neutrally charged.

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Urinary tract infections (UTIs) are caused mainly by uropathogenic (UPEC), accounting for both uncomplicated (75%) and complicated (65%) UTIs. Detecting UPEC in a specific, rapid, and timely manner is essential for eradication, and optical biosensors may be useful tools for detecting UPEC. Recently, biosensors have been developed for the selective detection of antigen-antibody-specific interactions.

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Microsporum canis, one of the most widespread dermatophytes worldwide, is a zoonotic microorganism that transmits infection from reservoirs such as cats and dogs to humans. This microorganism is associated with Tinea corporis and other clinical manifestations; however, few studies have used genetic surveillance to determine and characterize the process of zoonotic transmission. In this study, we show a clear example of zoonotic transmission from a cat to an intrafamilial environment, where it caused Tinea corporis by infection with M.

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is a widely distributed dermatophyte, which is among the main etiological agents of dermatophytosis in humans and domestic animals. This fungus invades, colonizes and nourishes itself on the keratinized tissues of the host through various virulence factors. This review will bring together the known information about the mechanisms, enzymes and their associated genes relevant to the pathogenesis processes of the fungus and will provide an overview of those virulence factors that should be better studied to establish effective methods of prevention and control of the disease.

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  • A comprehensive analysis of 356 Klebsiella pneumoniae species complex isolates revealed three main types: classical (cl), presumptive hypervirulent (p-hv), and hypermucoviscous-like (hmv-like), with the majority being classical at 82.3%.
  • Most classical isolates produced extended-spectrum-β-lactamases (ESBLs), and a small percentage showed colistin resistance, while all p-hv strains were antibiotic-susceptible.
  • The study found significant differences in capsule production between the different types, which affected virulence, and highlighted the emergence of atypical hypervirulent strains in the population, emphasizing the importance of tracking these pathogens in Mexican healthcare settings.
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The present study evaluates the effects of vaccination with Brucella melitensis strains Rev 1 ΔeryCD and Rev 1 on the reproductive system of male goats. Three groups, each of them consisting of 15 six-month-old brucellosis-free male goats, were studied. The first group was vaccinated with the Rev 1 ΔeryCD strain, the second group received Rev 1 and the third group was inoculated with sterile physiological saline solution.

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Introduction: Recurrent urinary tract infections (RUTIs) caused by uropathogenic are costly public health problems impacting patients' quality of life.

Aim: In this work, a comparative genomics analysis of three clinical RUTI strains isolated from bladder biopsy specimens was performed.

Materials And Methods: One hundred seventy-two whole genomes of urinary tract strains were selected from the NCBI database.

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Chromoblastomycosis is a chronic granulomatous mycosis of the skin and subcutaneous tissue caused by traumatic inoculation with dematiaceous fungi. This disease primarily affects agricultural workers, who are mostly men. We present a case of chromoblastomycosis in a 63-year-old male farmer patient with dermatosis over 50 years of evolution, with warty, erythematous, and scaly plaques that predominate on the left hemithorax.

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978-A_19 was obtained from a parrot with pneumonia. It is resistant to ampicillin, carbenicillin, cephalosporins, clindamycin, and trimethoprim + sulfamethoxazole. The genome encodes a new ADC allele, a OXA-502 gene, possesses several virulence genes related to adherence and biofilm formation, and has types I, II, and IV secretion systems.

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Objective: We present a case of primary cutaneous mucormycosis in a patient with bone marrow failure secondary to paroxysmal nocturnal haemoglobinuria (PNH).

Clinical Case: A 60-year-old male patient with a history of PNH, complicated to a severe aplastic anaemia, presented to the emergency department complaining of papules on the lower limbs that rapidly turned into necrotic plaques within 2 months. Histopathological examination showed granulomatous and suppurative dermatitis with tissue necrosis and the presence of non-septate hyphae.

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This study evaluated the antagonistic effect of the JLM strain isolated from , against RB51, S19, and 2308 strains, during the manufacture of soft-ripened cheese. First, the tolerance of JLM was tested with pH values and bile salt concentrations for 3 h to simulate digestive tract conditions. The antagonistic effect against strains was evaluated through double-layer diffusion and agar well diffusion assays.

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Unlabelled: The CS21 pilus produced by enterotoxigenic (ETEC) is involved in adherence to HT-29 intestinal cells. The CS21 pilus assembles proteins encoded by 14 genes clustered into the operon.

Aim: This study aimed to determine whether .

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Enterococci exhibit clumping under the selective pressure of antibiotics. The aim of this study was to analyze the effect of supernatants from a plasmid-free clone (C29) of subjected to 0.25×, 0.

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There have been few reports on extra-enteric infections by Blastocystis STs and none have been molecularly identified in samples from human reproductive organs. We report for the first time the identification of 3 different subtypes of Blastocystis (ST1-3) in vaginal and sperm samples, from patients infected with Trichomonas vaginalis. Blastocystis STs were identified by PCR-sequencing and by phylogenetic inferences using 28 vaginal swab samples and 7 sperm samples from patients trichomoniasis.

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  • Sporotrichosis is a fungal infection affecting humans, primarily caused by various species of the Sporothrix genus, with the aim of this study being to analyze its prevalence in the Americas over the last decade.* -
  • The research involved an extensive search of medical articles from 2012 to 2022, revealing 124 studies related to 12,568 patients, with the majority of cases reported in South America (87.38%), particularly in Brazil, Peru, and Mexico.* -
  • Findings indicate a higher prevalence in regions with conducive environmental factors and zoonotic transmission, while the most common infection type was lymphocutaneous; however, molecular diagnostic methods remain underdeveloped due to costs.*
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