Publications by authors named "Henryk Korza"
Article Synopsis
- The resistance of pests to insecticides is a growing global problem that impacts food production, particularly with widely used diamide insecticides which target insect ryanodine receptors (RyRs).
- Using advanced techniques like chimeric RyR and cryo-electron microscopy, researchers studied how different diamide insecticides interact with RyRs and how this relates to emerging resistance mutations.
- The study reveals how these resistance mutations change the receptor's structure, reducing the effectiveness of insecticides, and offers insights for developing new pesticides that can overcome these resistance challenges.
Ryanodine receptor (RyR) is a giant calcium release channel located on the membrane of the endoplasmic reticulum (ER). Here, we report the regulation of RyRs from two major agricultural pests, diamondback moth and fall armyworm, by insect calmodulin (CaM). The recombinantly expressed full-length insect RyR could be pulled down by insect CaM in the presence of Ca, but the efficiency is lower compared to rabbit RyR1 and insect RyR with the CaM-binding domain (CaMBD) replaced by rabbit RyR1 sequence.
View Article and Find Full Text PDFRestriction endonucleases naturally target DNA duplexes. Systematic screening has identified a small minority of these enzymes that can also cleave RNA/DNA heteroduplexes and that may therefore be useful as tools for RNA biochemistry. We have chosen AvaII (G↓GWCC, where W stands for A or T) as a representative of this group of restriction endonucleases for detailed characterization.
View Article and Find Full Text PDFTetraspanins are integral membrane proteins that function as organizers of multimolecular complexes and modulate function of associated proteins. Mammalian genomes encode approximately 30 different members of this family and remotely related eukaryotic species also contain conserved tetraspanin homologs. Tetraspanins are involved in a number of fundamental processes such as regulation of cell migration, fusion, immunity and signaling.
View Article and Find Full Text PDFPhotosystem II from transplastomic plants of Nicotiana tabacum with a hexahistidine tag at the N-terminal end of the PsbE subunit (α-chain of the cytochrome b(559)) was purified according to the protocol of Fey et al. (BBA 12:1501-1509, 2008). The protein sample was then subjected to two additional gel filtration runs in order to increase its homogeneity and to standardize the amount of detergent.
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